BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5455

Title: 1H, 13C and 15N resonance assignments of the human phosphatase PRL-3   PubMed: 12495037

Authors: Kozlov, Guennadi; Cheng, Jing; Lievre, Carine; Banville, Denis; Gehring, Kalle; Ekiel, Irena

Citation: Kozlov, Guennadi; Cheng, Jing; Lievre, Carine; Banville, Denis; Gehring, Kalle; Ekiel, Irena. "Letter to the Editor: 1H, 13C and 15N Resonance Assignments of the Human Phosphatase PRL-3"  J. Biomol. NMR 24, 169-170 (2002).

Assembly members:
PRL-3, polymer, 172 residues, 19383 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PRL-3: GSHMARMNRPAPVEVSYKHM RFLITHNPTNATLSTFIEDL KKYGATTVVRVCEVTYDKTP LEKDGITVVDWPFDDGAPPP GKVVEDWLSLVKAKFCEAPG SCVAVHCVAGLGRAPVLVAL ALIESGMKYEDAIQFIRQKR RGAINSKQLTYLEKYRPKQR LRFKDPHTHKTR

Data sets:
Data typeCount
13C chemical shifts588
15N chemical shifts149
1H chemical shifts911

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PRL-3 subunit 11

Entities:

Entity 1, PRL-3 subunit 1 172 residues - 19383 Da.

1   GLYSERHISMETALAARGMETASNARGPRO
2   ALAPROVALGLUVALSERTYRLYSHISMET
3   ARGPHELEUILETHRHISASNPROTHRASN
4   ALATHRLEUSERTHRPHEILEGLUASPLEU
5   LYSLYSTYRGLYALATHRTHRVALVALARG
6   VALCYSGLUVALTHRTYRASPLYSTHRPRO
7   LEUGLULYSASPGLYILETHRVALVALASP
8   TRPPROPHEASPASPGLYALAPROPROPRO
9   GLYLYSVALVALGLUASPTRPLEUSERLEU
10   VALLYSALALYSPHECYSGLUALAPROGLY
11   SERCYSVALALAVALHISCYSVALALAGLY
12   LEUGLYARGALAPROVALLEUVALALALEU
13   ALALEUILEGLUSERGLYMETLYSTYRGLU
14   ASPALAILEGLNPHEILEARGGLNLYSARG
15   ARGGLYALAILEASNSERLYSGLNLEUTHR
16   TYRLEUGLULYSTYRARGPROLYSGLNARG
17   LEUARGPHELYSASPPROHISTHRHISLYS
18   THRARG

Samples:

sample_1: PRL-3, [U-15N; U-13C], 2.5 – 3.0 mM

cond1: ionic strength: 0.15 M; pH: 6.8; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available

Software:

XEASY - assignments

GIFA - processing

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 19395
PDB
DBJ BAJ20896
GB AAC29314 EHH25482 EHH64453
REF NP_001180623 NP_116000 XP_002819531 XP_003819536 XP_003903255
SP O75365