BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5335

Title: Backbone and side chain 1H, 13C, and 15N chemical shift assignments for E.coli protein YacG   PubMed: 12211008

Authors: Ramelot, Theresa; Kennedy, Michael

Citation: Ramelot, Theresa; Cort, J.; Yee, A.; Semesi, A.; Edwards, A.; Arrowsmith, C.; Kennedy, Michael. "NMR Structure of the Eschericia coli Protein YacG: A Novel Sequence Motif in the Zinc-finger Family of Proteins "  Proteins 49, 289-293 (2002).

Assembly members:
YacG, polymer, 68 residues, 7306 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Eschericia coli

Experimental source:   Production method: recombinant technology   Host organism: Eschericia coli

Entity Sequences (FASTA):
YacG: GSHMSETITVNCPTCGKTVV WGEISPFRPFCSKRCQLIDL GEWAAEEKRIPSSGDLSESD DWSEEPKQ

Data sets:
Data typeCount
13C chemical shifts256
1H chemical shifts385
15N chemical shifts61

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YacG1
2zinc ion2

Entities:

Entity 1, YacG 68 residues - 7306 Da.

1   GLYSERHISMETSERGLUTHRILETHRVAL
2   ASNCYSPROTHRCYSGLYLYSTHRVALVAL
3   TRPGLYGLUILESERPROPHEARGPROPHE
4   CYSSERLYSARGCYSGLNLEUILEASPLEU
5   GLYGLUTRPALAALAGLUGLULYSARGILE
6   PROSERSERGLYASPLEUSERGLUSERASP
7   ASPTRPSERGLUGLUPROLYSGLN

Entity 2, zinc ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: YacG, [U-13C; U-15N], 1 mM; phosphate buffer 25 mM; NaCl 450 mM

sample_2: YacG, [U-15N], 1 – 3 mM; phosphate buffer 25 mM; NaCl 450 mM

cond_1: pH: 6.5 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablecond_1
1H-13C HSQC (aliph)not availablenot availablecond_1
15N-edited NOESY-HQSCnot availablenot availablecond_1
HCC-TOCSY-NNHnot availablenot availablecond_1
HCCH-TOCSYnot availablenot availablecond_1
CN-NOESY-HSQCnot availablenot availablecond_1
HNCOnot availablenot availablecond_1
HNCACBnot availablenot availablecond_1
CBCA(CO)NHnot availablenot availablecond_1
CCC-TOCSY-NNHnot availablenot availablecond_1
HNHAnot availablenot availablecond_1
4D CC-NOESYnot availablenot availablecond_1
1H-13C HSQC (alpha)not availablenot availablecond_1
1H-13C HSQC (arom)not availablenot availablecond_1

Software:

Felix v98 - processing data

XPLOR v3.84 - structure calculations

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 750 MHz
  • Varian Inova 800 MHz

Related Database Links:

SWISS-PROT P0A8H8 P0A8H9 A7ZW52 B1IR78 A7ZHJ2
REF NP_706056 NP_308132 NP_414643 AP_000763 NP_285797
GenBank AAN78619 AAP15644 AAG54405 AAN41763 AAC73212
DBJ BAB96668 BAB33528
PDB