BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5327

Title: Structural basis for Hif-1alpha/CBP recognition in the cellular hypoxic response   PubMed: 11959977

Authors: Dames, Sonja; Martinez-Yamout, Maria; De Guzman, Roberto; Dyson, H. Jane; Wright, Peter

Citation: Dames, Sonja; Martinez-Yamout, Maria; De Guzman, Roberto; Dyson, H.; Wright, Peter. "From the Cover: Structural basis for Hif-1alpha/CBP Recognition in the Cellular Hypoxic Response"  Proc. Natl. Acad. Sci. U.S.A. 99, 5271-5276 (2002).

Assembly members:
hypoxia induced factor 1 alpha, polymer, 51 residues, Formula weight is not available
CREB-binding protein, polymer, 95 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
hypoxia induced factor 1 alpha: SDLACRLLGQSMDESGLPQL TSYDCEVNAPIQGSRNLLQG EELLRALDQVN
CREB-binding protein: ADPEKRKLIQQQLVLLLHAH KCQRREQANGEVRACSLPHC RTMKNVLNHMTHCQAGKACQ VAHCASSRQIISHWKNCTRH DCPVCLPLKNASDKR

Data sets:
Data typeCount
1H chemical shifts939
13C chemical shifts467
15N chemical shifts154

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hif-1alpha1
2CBP2
3Zinc ion, I3
4Zinc ion, II3
5Zinc ion, III3

Entities:

Entity 1, Hif-1alpha 51 residues - Formula weight is not available

1   SERASPLEUALACYSARGLEULEUGLYGLN
2   SERMETASPGLUSERGLYLEUPROGLNLEU
3   THRSERTYRASPCYSGLUVALASNALAPRO
4   ILEGLNGLYSERARGASNLEULEUGLNGLY
5   GLUGLULEULEUARGALALEUASPGLNVAL
6   ASN

Entity 2, CBP 95 residues - Formula weight is not available

1   ALAASPPROGLULYSARGLYSLEUILEGLN
2   GLNGLNLEUVALLEULEULEUHISALAHIS
3   LYSCYSGLNARGARGGLUGLNALAASNGLY
4   GLUVALARGALACYSSERLEUPROHISCYS
5   ARGTHRMETLYSASNVALLEUASNHISMET
6   THRHISCYSGLNALAGLYLYSALACYSGLN
7   VALALAHISCYSALASERSERARGGLNILE
8   ILESERHISTRPLYSASNCYSTHRARGHIS
9   ASPCYSPROVALCYSLEUPROLEULYSASN
10   ALASERASPLYSARG

Entity 3, Zinc ion, I - Zn - 65.409 Da.

1   ZN

Samples:

Sample_1: hypoxia induced factor 1 alpha 2 mM; CREB-binding protein, [U-13C; U-15N], 1 mM; ZINC (II) ION 3 mM; D-TRIS 10 mM; D-DTT 10 mM; NaN3 0.02%; ZNSO4 0.5 mM; H2O 90%; D2O 10%

Sample_2: hypoxia induced factor 1 alpha 2 mM; CREB-binding protein, [U-13C; U-15N], 1 mM; ZINC (II) ION 3 mM; D-TRIS 10 mM; D-DTT 10 mM; NaN3 0.02%; ZNSO4 0.5 mM; D2O 100%

Sample_3: hypoxia induced factor 1 alpha 2 mM; CREB-binding protein, [U-15N], 1 mM; ZINC (II) ION 3 mM; D-TRIS 10 mM; D-DTT 10 mM; NaN3 0.02%; ZNSO4 0.5 mM; H2O 90%; D2O 10%

Sample_4: hypoxia induced factor 1 alpha, [U-13C; U-15N], 1 mM; CREB-binding protein 2 mM; ZINC (II) ION 6 mM; D-TRIS 10 mM; D-DTT 10 mM; NaN3 0.02%; ZNSO4 0.5 mM; H2O 90%; D2O 10%

Sample_5: hypoxia induced factor 1 alpha, [U-13C; U-15N], 1 mM; CREB-binding protein 2 mM; ZINC (II) ION 6 mM; D-TRIS 10 mM; D-DTT 10 mM; NaN3 0.02%; ZNSO4 0.5 mM; D2O 100%

Sample_6: hypoxia induced factor 1 alpha, [U-15N], 1 mM; CREB-binding protein 2 mM; ZINC (II) ION 6 mM; D-TRIS 10 mM; D-DTT 10 mM; NaN3 0.02%; ZNSO4 0.5 mM; H2O 90%; D2O 10%

cond_1: pH: 6.1; temperature: 298 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablecond_1
1H-13C NOESYnot availablenot availablecond_1

Software:

No software information available

NMR spectrometers:

  • BRUKER DRX 800 MHz
  • BRUKER DRX 600 MHz

Related Database Links:

BMRB 16014 18650 5987 6268 5987 6268
PDB
DBJ BAE06125 BAG65526 BAI45616 BAG65526 BAE06125
EMBL CAF96470 CAG06081
GB AAB28651 AAC08447 AAC51331 AAC51770 AAH44677
PRF 1923401A 1923401A
REF NP_001020603 NP_001073315 NP_001088637 NP_001157494 NP_001247644 NP_596872 XP_001095225 NP_001073315 NP_004371 NP_001020603
SP P45481 Q6JHU9 Q92793
TPG DAA15549
SWISS-PROT P45481 Q92793
GenBank AAC51770 AAH72594 AAC08447 AAC51331 AAB28651