BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5293

Title: NMR Identification and Characterization of the Flexible Regions in the 160 KD Molten Globule-like Aggregate of Barstar at Low pH   PubMed: 12146954

Authors: Juneja, Juhi; Bhavesh, Neel; Udgaonkar, Jayant; Hosur, Ramakrishna

Citation: Juneja, Juhi; Bhavesh, Neel; Udgaonkar, Jayant; Hosur, Ramakrishna. "NMR Identification and Characterization of the Flexible Regions in the 160 kDa Molten Globule-like Aggregate of Barstar at low pH"  Biochemistry 41, 9885-9899 (2002).

Assembly members:
Barstar, polymer, 89 residues, 10100 Da.

Natural source:   Common Name: Bacillus amyloliquefaciens   Taxonomy ID: 1390   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus amyloliquefaciens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Barstar: KKAVINGEQIRSISDLHQTL KKELALPEYYGENLDALWDC LTGWVEYPLVLEWRQFEQSK QLTENGAESVLQVFREAKAE GCDITIILS

Data sets:
Data typeCount
1H chemical shifts131
13C chemical shifts47
15N chemical shifts21
coupling constants17

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1barstar aggegate 11
2barstar aggegate 21
3barstar aggegate 31
4barstar aggegate 41
5barstar aggegate 51
6barstar aggegate 61
7barstar aggegate 71
8barstar aggegate 81
9barstar aggegate 91
10barstar aggegate 101
11barstar aggegate 111
12barstar aggegate 121
13barstar aggegate 131
14barstar aggegate 141
15barstar aggegate 151
16barstar aggegate 161

Entities:

Entity 1, barstar aggegate 1 89 residues - 10100 Da.

1   LYSLYSALAVALILEASNGLYGLUGLNILE
2   ARGSERILESERASPLEUHISGLNTHRLEU
3   LYSLYSGLULEUALALEUPROGLUTYRTYR
4   GLYGLUASNLEUASPALALEUTRPASPCYS
5   LEUTHRGLYTRPVALGLUTYRPROLEUVAL
6   LEUGLUTRPARGGLNPHEGLUGLNSERLYS
7   GLNLEUTHRGLUASNGLYALAGLUSERVAL
8   LEUGLNVALPHEARGGLUALALYSALAGLU
9   GLYCYSASPILETHRILEILELEUSER

Samples:

sample_1: Barstar, [U-90% 15N], 1.2 mM

sample_2: Barstar, [U-95% 13C; U-90% 15N], 1.2 mM

sample_3: Barstar 1.2 mM

Cond_1: pH: 2.7 na; temperature: 298 K; pressure: 760 mmHg

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNNnot availablenot availablenot available
HN(C)Nnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available
1H-15N HSQC-TOCSYnot availablenot availablenot available
1H-15N TOCSY-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
1H-1H NOESYnot availablenot availablenot available

Software:

Felix v97 - data processing, resonance assignment

NMR spectrometers:

  • Varian Unityplus 600 MHz

Related Database Links:

BMRB 6227 7126
PDB
EMBL CAA33551 CBI41926 CCG48789 CCP20814 CDG25099
GB AAM10782 AAO92245 AAP41138 AAP41140 ABS73233
REF WP_007408543 WP_007610138 WP_013351425 WP_014720882 WP_015239422
SP P11540