BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5282

Title: Refinement of d(GCGAAGC) Hairpin Structure Using One- and Two-Bonds Residual Dipolar Couplings   PubMed: 12449414

Authors: Padrta, P.; Stefl, R.; Zidek, L.; Sklenar, V.

Citation: Padrta, P.; Stefl, R.; Kralik, L.; Zidek, L.; Sklenar, V.. "Refinement of d(GCGAAGC) Hairpin Structure Using One- and Two-bond Residual Dipolar Couplings"  J. Biomol. NMR 24, 1-14 (2002).

Assembly members:
GCGAAGC hairpin, polymer, 7 residues, Formula weight is not available

Natural source:   Common Name: Herpes simplex virus   Taxonomy ID: 10294   Superkingdom: Viruses   Kingdom: not available   Genus/species: Simplexvirus n/a

Experimental source:   Production method: vendor

Entity Sequences (FASTA):
GCGAAGC hairpin: GCGAAGC

Data sets:
Data typeCount
13C chemical shifts35
15N chemical shifts9
1H chemical shifts68
31P chemical shifts6

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
15'-D(*GP*CP*GP*AP*AP*GP*C)-3'1

Entities:

Entity 1, 5'-D(*GP*CP*GP*AP*AP*GP*C)-3' 7 residues - Formula weight is not available

1   DGDCDGDADADGDC

Samples:

sample_1: GCGAAGC hairpin 6 mM; Na phosphate buffer 10 mM; D2O 100%

sample_2: GCGAAGC hairpin 6 mM; Na phosphate buffer 10 mM; H2O 90%; D2O 10%

sample_3: GCGAAGC hairpin, [U-13C; U-15N], 0.5 mM; Na phosphate buffer 10 mM; H2O 90%; D2O 10%

condition_1: pH: 6.7; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablecondition_1
E-COSYnot availablenot availablecondition_1
DQF-COSYnot availablenot availablecondition_1
COUPLED HSQCnot availablenot availablecondition_1
S3E E-COSYnot availablenot availablecondition_1

Software:

xwinnmr v2.6,3.0 - spectra processing

SPARKY v3.xx - assignment and integration

AMBER v6.0 - structure refinement

NMR spectrometers:

  • Bruker AVANCE-DRX 500 MHz

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