BMRB Entry 52255
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52255
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NMR-STAR v3 text file.
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Citation: Luo, Yingyue; Theillet, Francois-Xavier. "Structural characterization of the MDM2 NLS/NES/arrestin-binding/acidic domain in phospho- and unmodified-forms" .
Assembly members:
entity_1, polymer, 15 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: XSSSEXTGXPSNPDX
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 11 |
| 1H chemical shifts | 49 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mdm2aa211-223_pS215-pT218 | 1 |
Entities:
Entity 1, Mdm2aa211-223_pS215-pT218 15 residues - Formula weight is not available
Ac-SSSEpSTGpTPSNPD-NH2
| 1 | ACE | SER | SER | SER | GLU | SEP | THR | GLY | TPO | PRO | ||||
| 2 | SER | ASN | PRO | ASP | NH2 |
Samples:
sample_1: Mdm2aa211_223_pS215_pT218 3 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DSS 0.5 mM
sample_conditions_1: ionic strength: 0.190 M; pH: 7.1; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H ROESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4 - collection
TOPSPIN v3 - collection
CcpNMR v3 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 700 MHz
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks