BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5224

Title: 1H, 13C and 15N Chemical shifts assignments for the C-terminal domain (region 428-547) of human lamin A/C   PubMed: 12018485

Authors: Krimm, Isabelle; Couprie, Joel; Ostlund, Cecilia; Worman, Howard; Zinn-Justin, Sophie

Citation: Krimm, Isabelle; Couprie, Joel; Ostlund, Cecilia; Worman, Howard; Zinn-Justin, Sophie. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the C-terminal domain of human lamin A/C "  J. Biomol. NMR 22, 371-372 (2002).

Assembly members:
lamin A/C, polymer, 120 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
lamin A/C: SSFSQHARTSGRVAVEEVDE EGKFVRLRNKSNEDQSMGNW QIKRQNGDDPLLTYRFPPKF TLKAGQVVTIWAAGAGATHS PPTDLVWKAQNTWGCGNSLR TALINSTGEEVAMRKLVRSV

Data sets:
Data typeCount
1H chemical shifts802
13C chemical shifts492
15N chemical shifts127

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lamin1

Entities:

Entity 1, lamin 120 residues - Formula weight is not available

1   SERSERPHESERGLNHISALAARGTHRSER
2   GLYARGVALALAVALGLUGLUVALASPGLU
3   GLUGLYLYSPHEVALARGLEUARGASNLYS
4   SERASNGLUASPGLNSERMETGLYASNTRP
5   GLNILELYSARGGLNASNGLYASPASPPRO
6   LEULEUTHRTYRARGPHEPROPROLYSPHE
7   THRLEULYSALAGLYGLNVALVALTHRILE
8   TRPALAALAGLYALAGLYALATHRHISSER
9   PROPROTHRASPLEUVALTRPLYSALAGLN
10   ASNTHRTRPGLYCYSGLYASNSERLEUARG
11   THRALALEUILEASNSERTHRGLYGLUGLU
12   VALALAMETARGLYSLEUVALARGSERVAL

Samples:

sample_1: lamin A/C, [U-13C; U-15N], 0.5 – 1.5 mM

Ex-cond_1: pH*: 6.3; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available

Software:

NMR-Pipe -

Felix -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ BAD51965 BAE72960 BAE87519 BAF62394 BAG57524
EMBL CAA27173 CAA27174 CAJ28141
GB AAA36160 AAA36164 AAH00511 AAH03162 AAH14507
PRF 1203377A
REF NP_001029225 NP_001092024 NP_001104727 NP_001244303 NP_001244822
SP P02545 Q3ZD69
TPG DAA31761