BMRB Entry 52154

Title:
Endo-b-1,4-xylanase (Xylanase A) WT from Bacillus subtilis Lipari-Szabo order parameters and relaxation data
Deposition date:
2023-09-28
Original release date:
2023-10-18
Authors:
MacDonald, Meagan
Citation:

Citation: MacDonald, Meagan; Wells, Nicholas; Hassan, Bakar; Dudley, Joshua; Walters, Kylie; Korzhnev, Dmitry; Aramini, James; Smith, Colin. "Effects of Xylanase A double mutation on substrate specificity and structural dynamics"  J. Struct. Biol. 216, 108082-108082 (2024).
PubMed: 38438058

Assembly members:

Assembly members:
entity_1, polymer, 189 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
T1 relaxation values175
T1rho relaxation values175
T2 relaxation values175
heteronuclear NOE values175
order parameters169

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Xylanase A1

Entities:

Entity 1, Xylanase A 189 residues - Formula weight is not available

His-tag scar GSHM included in the sequence

1   GLYSERHISMETALASERTHRASPTYRTRP
2   GLNASNTRPTHRASPGLYGLYGLYILEVAL
3   ASNALAVALASNGLYSERGLYGLYASNTYR
4   SERVALASNTRPSERASNTHRGLYASNPHE
5   VALVALGLYLYSGLYTRPTHRTHRGLYSER
6   PROPHEARGTHRILEASNTYRASNALAGLY
7   VALTRPALAPROASNGLYASNGLYTYRLEU
8   THRLEUTYRGLYTRPTHRARGSERPROLEU
9   ILEGLUTYRTYRVALVALASPSERTRPGLY
10   THRTYRARGPROTHRGLYTHRTYRLYSGLY
11   THRVALLYSSERASPGLYGLYTHRTYRASP
12   ILETYRTHRTHRTHRARGTYRASNALAPRO
13   SERILEASPGLYASPARGTHRTHRPHETHR
14   GLNTYRTRPSERVALARGGLNSERLYSARG
15   PROTHRGLYSERASNALATHRILETHRPHE
16   SERASNHISVALASNALATRPLYSSERHIS
17   GLYMETASNLEUGLYSERASNTRPALATYR
18   GLNVALMETALATHRGLUGLYTYRGLNSER
19   SERGLYSERSERASNVALTHRVALTRP

Samples:

sample_1: Xylanase A, [U-100% 15N], 0.64 mM; sodium acetate 25 mM; sodium chloride 50 mM; D2O, [U-100% 2H], 10%; sodium azide 0.03%

sample_conditions_1: ionic strength: 0.05 M; pH: 5.7; pressure: 1 atm; temperature: 303.30 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N T1sample_1isotropicsample_conditions_1
2D 1H-15N T1rhosample_1isotropicsample_conditions_1
2D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

VNMRj v4.0 - collection

NMRDraw - processing

FitNMR v0.4 - data analysis

FASTModelFree v1.3 - data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz