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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5212
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Hwang, P.; Li, C.; Morra, M.; Lillywhite, J.; Muhandiram, D.; Gertler, F.; Terhorst, C.; Kay, L.; Pawson, T.; Forman-Kay, J.; Li, S.-C.. "A "three-pronged" Binding Mechanism for the SAP/SH2D1A SH2 Domain: Structural
Basis and Relevance to the XLP Syndrome" EMBO J. 21, 314-323 (2002).
PubMed: 11823424
Assembly members:
SAP/SH2D1A, polymer, 128 residues, Formula weight is not available
peptide n-pY, polymer, 9 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SAP/SH2D1A: MDAVAVYHGKISRETGEKLL
LATGLDGSYLLRDSESVPGV
YCLCVLYHGYIYTYRVSQTE
TGSWSAETAPGVHKRYFRKI
KNLISAFQKPDQGIVIPLQY
PVEKKSSARSTQGTTGIRED
PDVCLKAP
peptide n-pY: RKSLTIXAX
Data type | Count |
1H chemical shifts | 773 |
15N chemical shifts | 133 |
13C chemical shifts | 557 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | SH2 DOMAIN PROTEIN 1A | 1 |
2 | peptide n-pY | 2 |
Entity 1, SH2 DOMAIN PROTEIN 1A 128 residues - Formula weight is not available
1 | MET | ASP | ALA | VAL | ALA | VAL | TYR | HIS | GLY | LYS | ||||
2 | ILE | SER | ARG | GLU | THR | GLY | GLU | LYS | LEU | LEU | ||||
3 | LEU | ALA | THR | GLY | LEU | ASP | GLY | SER | TYR | LEU | ||||
4 | LEU | ARG | ASP | SER | GLU | SER | VAL | PRO | GLY | VAL | ||||
5 | TYR | CYS | LEU | CYS | VAL | LEU | TYR | HIS | GLY | TYR | ||||
6 | ILE | TYR | THR | TYR | ARG | VAL | SER | GLN | THR | GLU | ||||
7 | THR | GLY | SER | TRP | SER | ALA | GLU | THR | ALA | PRO | ||||
8 | GLY | VAL | HIS | LYS | ARG | TYR | PHE | ARG | LYS | ILE | ||||
9 | LYS | ASN | LEU | ILE | SER | ALA | PHE | GLN | LYS | PRO | ||||
10 | ASP | GLN | GLY | ILE | VAL | ILE | PRO | LEU | GLN | TYR | ||||
11 | PRO | VAL | GLU | LYS | LYS | SER | SER | ALA | ARG | SER | ||||
12 | THR | GLN | GLY | THR | THR | GLY | ILE | ARG | GLU | ASP | ||||
13 | PRO | ASP | VAL | CYS | LEU | LYS | ALA | PRO |
Entity 2, peptide n-pY 9 residues - Formula weight is not available
1 | ARG | LYS | SER | LEU | THR | ILE | PTR | ALA | NH2 |
sample_1: SAP/SH2D1A, [U-15N; U-13C], 1 mM; peptide n-pY 1 mM; phosphate buffer 20 mM; NaCl 100 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 6.0; temperature: 303 K; ionic strength: 120 mM; pressure: 1 atm
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HN(CO)HB | sample_1 | not available | sample_cond_1 |
3D HNHB | sample_1 | not available | sample_cond_1 |
3D 15N,13C-separated NOESY | sample_1 | not available | sample_cond_1 |
ARIA v1.0 - structure solution
NMRPipe v1.8 - processing
NMRView v3.0 - data analysis
REF | NP_002342.1 XP_865096.1 NP_001028009.1 |
SWISS-PROT | O60880 |
GenBank | AAK11578.1 AAX37129.1 AAC62631.1 AAH20732.1 AAC62630.1 |
EMBL | CAG46840.1 CAA19222.1 CAG46828.1 CAA18777.1 |
PDB | |
BMRB | 5211 |
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