BMRB Entry 52082

Title:
D12RNAbound_TRBP2_DSRBD2 dynamics data
Deposition date:
2023-08-15
Original release date:
2023-09-08
Authors:
Parvez, Firdousi
Citation:

Citation: Parvez, Firdousi. "Differential conformational dynamics in two type-A RNA-binding domains drive the double-stranded RNA recognition and binding"  .

Assembly members:

Assembly members:
entity_1, polymer, 84 residues, Formula weight is not available
entity_2, polymer, 12 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHMGWA

Entity Sequences (FASTA):

Data typeCount
T1 relaxation values110
T2 relaxation values109
heteronuclear NOE values106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TRBP2-dsRBD21
2D12RNA2

Entities:

Entity 1, TRBP2-dsRBD2 84 residues - Formula weight is not available

1   SERASNALAGLNGLNSERGLUCYSASNPRO
2   VALGLYALALEUGLNGLULEUVALVALGLN
3   LYSGLYTRPARGLEUPROGLUTYRTHRVAL
4   THRGLNGLUSERGLYPROALAHISARGLYS
5   GLUPHETHRMETTHRCYSARGVALGLUARG
6   PHEILEGLUILEGLYSERGLYTHRSERLYS
7   LYSLEUALALYSARGASNALAALAALALYS
8   METLEULEUARGVALHISTHRVALPROLEU
9   ASPALAARGASP

Entity 2, D12RNA 12 residues - Formula weight is not available

1   CGUAAAUAUU
2   CG

Samples:

sample_1: TRBP2-dsRBD2 (TAR RNA binding protein isoform 2, second dsRNA binding domain), [U-15N], 1 mM; D12_RNA 50 uM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N heteronoesample_1isotropicsample_conditions_1
1H-15N heteronoesample_1isotropicsample_conditions_1
T1/R1 relaxationsample_1isotropicsample_conditions_1
T2/R2 relaxationsample_1isotropicsample_conditions_1
T1rho/R1rho relaxationsample_1isotropicsample_conditions_1
T2rho/R2rho relaxationsample_1isotropicsample_conditions_1
T1/R1 relaxationsample_1isotropicsample_conditions_1
T2/R2 relaxationsample_1isotropicsample_conditions_1

Software:

SPARKY - peak picking

Mathematica - data analysis

NMRPipe - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz