BMRB Entry 51683

Title:
UGT2B17-C terminus
Deposition date:
2022-11-01
Original release date:
2023-02-02
Authors:
Sulekha, Anamika; Osborne, Michael; Gasiorek, Jadwiga; Borden, Katherine
Citation:

Citation: Sulekha, Anamika; Osborne, Michael; Gasiorek, Jadwiga; Borden, Katherine. "1H, 13C, 15N Backbone and sidechain chemical shift assignments of the C-terminal domain of human UDP-glucuronosyltransferase 2B17 (UGT2B17-C)"  Biomol. NMR Assignments 17, 67-73 (2023).
PubMed: 36757531

Assembly members:

Assembly members:
entity_1, polymer, 177 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28a

Data sets:
Data typeCount
13C chemical shifts641
15N chemical shifts145
1H chemical shifts760

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UGT2B17-C1

Entities:

Entity 1, UGT2B17-C 177 residues - Formula weight is not available

1   GLYSERGLUPHEGLYLEUHISCYSLYSPRO
2   ALALYSPROLEUPROLYSGLUMETGLUGLU
3   PHEVALGLNSERSERGLYGLUASNGLYILE
4   VALVALPHESERLEUGLYSERMETILESER
5   ASNMETSERGLUGLUSERALAASNMETILE
6   ALASERALALEUALAGLNILEPROGLNLYS
7   VALLEUTRPARGPHEASPGLYLYSLYSPRO
8   ASNTHRLEUGLYSERASNTHRARGLEUTYR
9   LYSTRPLEUPROGLNASNASPLEULEUGLY
10   HISPROLYSTHRLYSALAPHEILETHRHIS
11   GLYGLYTHRASNGLYILETYRGLUALAILE
12   TYRHISGLYILEPROMETVALGLYILEPRO
13   LEUPHEALAASPGLNHISASPASNILEALA
14   HISMETLYSALALYSGLYALAALALEUSER
15   VALASPILEARGTHRMETSERSERARGASP
16   LEULEUASNALALEULYSSERVALILEASN
17   ASPPROILETYRLYSGLUASNILEMETLYS
18   LEUSERARGILEHISHISASP

Samples:

sample_1: UGT2B17-C, [U-13C; U-15N], 200 uM; D2O, [U-100% 2H], 7%; DTT, [U-2H], 1 mM; DSS, [U-2H], 1 mM; H2O 93%; sodium azide 0.02%

sample_conditions_1: ionic strength: 0.2 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

DANGLE - data analysis

TOPSPIN - collection

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks