BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5166

Title: Solution structure of hemolysin expression modulating protein Hha   PubMed: 11854485

Authors: Yee, A.; Chang, X.; Pineda-Lucena, A.; Wu, B.; Semesi, A.; Le, B.; Ramelot, T.; Lee, G.; Bhattacharyya, S.; Gutierrez, P.; Denisov, A.; Lee, C.; Cort, J.; Kozlov, G.; Liao, J.; Finak, G.; Chen, L.; Wishart, D.; Lee, W.; McIntosh, L.; Gehring, K.; Kennedy, M.; Edwards, A.; Arrowsmith, C.

Citation: Yee, A.; Chang, X.; Pineda-Lucena, A.; Wu, B.; Semesi, A.; Le, B.; Ramelot, T.; Lee, G.; Bhattacharyya, S.; Gutierrez, P.; Denisov, A.; Lee, C.; Cort, J.; Kozlov, G.; Liao, J.; Finak, G.; Chen, L.; Wishart, D.; Lee, W.; McIntosh, L.; Gehring, K.; Kennedy, M.; Edwards, A.; Arrowsmith, C.. "An NMR Approach to Structural Proteomics"  Proc. Natl. Acad. Sci. U.S.A. 99, 1825-1830 (2002).

Assembly members:
hemolysin expression modulating protein, polymer, 72 residues, Formula weight is not available

Natural source:   Common Name: E coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
hemolysin expression modulating protein: MSEKPLTKTDYLMRLRRCQT IDTLERVIEKNKYELSDNEL AVFYSAADHRLAELTMNKLY DKIPSSVWKFIR

Data sets:
Data typeCount
1H chemical shifts452
13C chemical shifts236
15N chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hemolysin expression modulating protein Hha1

Entities:

Entity 1, hemolysin expression modulating protein Hha 72 residues - Formula weight is not available

1   METSERGLULYSPROLEUTHRLYSTHRASP
2   TYRLEUMETARGLEUARGARGCYSGLNTHR
3   ILEASPTHRLEUGLUARGVALILEGLULYS
4   ASNLYSTYRGLULEUSERASPASNGLULEU
5   ALAVALPHETYRSERALAALAASPHISARG
6   LEUALAGLULEUTHRMETASNLYSLEUTYR
7   ASPLYSILEPROSERSERVALTRPLYSPHE
8   ILEARG

Samples:

sample_1: hemolysin expression modulating protein, [U-15N; U-13C], 2 mM; NaCl 150 mM

sample_cond_1: pH: 6.5; temperature: 298 K; ionic strength: 150 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D CN NOESYnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
CCC TOCSYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available
HCC TOCSYnot availablenot availablenot available
HNCACBnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available

Software:

NMRPipe v2000.02.14 - processing

Sparky v3.95 - data analysis

CNS v1.0 - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB
DBJ BAB33936 BAE76239 BAG76009 BAH62096 BAI23833
EMBL CAA41043 CAD04958 CAP74993 CAQ30933 CAQ90052
GB AAB40215 AAC73562 AAF67181 AAG10072 AAG54809
PIR AD0561
REF NP_286201 NP_308540 NP_414993 NP_455070 NP_459468
SP P0ACE3 P0ACE4 P0ACE5 P0ACE6