BMRB Entry 51599

Title:
hCEACAM1-Ig1-LBP4 Ala and Val methyl chemical shifts
Deposition date:
2022-08-28
Original release date:
2022-09-30
Authors:
Williams, Robert; Rogals, Monique; Eletsky, Alexander; Huang, Chin; Morris, Laura; Moremen, Kelley; Prestegard, James
Citation:

Citation: Williams, Robert; Rogals, Monique; Eletsky, Alexander; Huang, Chin; Morris, Laura; Moremen, Kelley; Prestegard, James. "AssignSLP_GUI, a software tool exploiting AI for NMR resonance assignment of sparsely labeled proteins"  J. Mag. Res. 345, 107336-107336 (2022).
PubMed: 36442299

Assembly members:

Assembly members:
entity_1, polymer, 124 residues, Formula weight is not available
entity_LU, non-polymer, 174.967 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: HEK293   Vector: pGEn2

Data sets:
Data typeCount
13C chemical shifts27
1H chemical shifts81

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hCEACAM1-Ig1-LBP41
2Lu3+2

Entities:

Entity 1, hCEACAM1-Ig1-LBP4 124 residues - Formula weight is not available

1   GLYSERGLYGLYALAGLNLEUTHRTHRGLU
2   SERMETPROPHEASNVALALAGLUGLYLYS
3   GLUVALLEULEULEUVALHISASNLEUPRO
4   GLNGLNLEUPHEGLYTYRSERTRPTYRLYS
5   GLYGLUARGVALASPGLYASNARGGLNILE
6   VALGLYTYRALACYSALAASPTHRASNASN
7   ASPGLYALATYRGLUGLYASPGLULEUCYS
8   GLNALATHRPROGLYPROALAASNSERGLY
9   ARGGLUTHRILETYRPROASNALASERLEU
10   LEUILEGLNASNVALTHRGLNASNASPTHR
11   GLYPHETYRTHRLEUGLNVALILELYSSER
12   ASPLEUVALASNGLUGLUALATHRGLYGLN
13   PHEHISVALTYR

Entity 2, Lu3+ - Lu - 174.967 Da.

1   LU

Samples:

sample_1: hCEACAM1-Ig1-LBP4, [CG1-Val, CG2-Val 90% 13C; CB-Ala 45% 13C], 300 uM; Luticium 300 uM; TRIS 25 mM; NaCl 100 mM; NaN3 0.02%; DSS 10 uM

sample_conditions_1: ionic strength: 125 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-1H HSQC (13C-deteected)sample_1isotropicsample_conditions_1

Software:

ASSIGN_SLP_GUI v2.4.7 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 900 MHz