BMRB Entry 5158

Title:
Conformational and Dynamic Characterization of the Molten Globule state of an Apomyoglobin Mutant with an altered Folding Pathway
Deposition date:
2001-09-25
Original release date:
2002-01-25
Authors:
Cavagnero, Silvia; Nishimura, Chiaki; Schwarzinger, Stephan; Dyson, Jane; Wright, Peter
Citation:

Citation: Cavagnero, Silvia; Nishimura, Chiaki; Schwarzinger, Stephan; Dyson, H.; Wright, Peter. "Conformational and Dynamic Characterization of the Molten Globule State of an Apomyoglobin Mutant with an Altered Folding Pathway"  Biochemistry 40, 14459-14467 (2001).
PubMed: 11724558

Assembly members:

Assembly members:
apomyoglobin, polymer, 153 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: sperm whale   Taxonomy ID: 9755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Physeter catodon

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts287
15N chemical shifts137
1H chemical shifts137

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apoMb GG mutant1

Entities:

Entity 1, apoMb GG mutant 153 residues - Formula weight is not available

1   VALLEUSERGLUGLYGLUTRPGLNLEUVAL
2   LEUHISVALTRPALALYSVALGLUALAASP
3   VALALAGLYHISGLYGLNASPILELEUILE
4   ARGLEUPHELYSSERHISPROGLUTHRLEU
5   GLULYSPHEASPARGPHELYSHISLEULYS
6   THRGLUALAGLUMETLYSALASERGLUASP
7   LEULYSLYSHISGLYVALTHRVALLEUTHR
8   ALALEUGLYALAILELEULYSLYSLYSGLY
9   HISHISGLUALAGLULEULYSPROLEUALA
10   GLNSERHISALATHRLYSHISLYSILEPRO
11   ILELYSTYRLEUGLUPHEILESERGLUALA
12   ILEILEHISVALLEUHISSERARGHISPRO
13   GLYASPPHEGLYALAASPALAGLNGLYALA
14   METGLYLYSALALEUGLYLEUPHEARGLYS
15   ASPILEALAALALYSTYRLYSGLULEUGLY
16   TYRGLNGLY

Samples:

sample_1: apomyoglobin, [U-13C; U-15N], 0.3 mM; ethanol, [U-2H], 10%; H2O 90%

cond1: pH: 4.1 na; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
(HCA)CO(CA)NHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available

Software:

NMRPipe -

NMRView -

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

BMRB 1027 1029 1200 1413 1455 1457 1459 1461 1463 1465 1467 1469 1471 15589 16217 16218 16499 16500 16501 1752 2345 2346 2347 2348 2431 2432 2433 2434 291 292 293 40 4061 4062 426 4568 4676 4695 823 824
PDB
DBJ BAF03579
GB AAA72199
PRF 742482A
REF NP_001277651
SP P02185
AlphaFold P02185

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks