BMRB Entry 51546

Name: Cadmium-loaded form of C. elegans MTL2
Published: 2024-01-11

Click here to enlarge.

PDB ID: 8ap5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51546
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Cadmium-loaded form of C. elegans MTL2

Deposition date:

2022-08-04

Original release date:

2024-01-11

Authors:

Leszczyszyn, Oksana; Blindauer, Claudia; Sturzenbaum, Stephen

Citation:

Citation: Essig, Yona; Leszczyszyn, Oksana; Almutairi, Nora; Harrison-Smith, Alexandra; Blease, Alix; Zeitoun-Ghandour, Sukaina; Webb, Sam; Blindauer, Claudia; Sturzenbaum, Stephen. "Metallothionein-mediated metal trafficking in the nematode C. elegans: dissection of structural and functional differentiation" .

Assembly members:

Assembly members:
entity_1, polymer, 63 residues, Formula weight is not available
entity_CD, non-polymer, 112.411 Da.

Natural source:

Natural source: Common Name: C. elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29a_mtl2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MVCKCDCKNQNCSCNTGTKD CDCSDAKCCEQYCCPTASEK KCCKSGCAGGCKCANCECAQ AAH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	308

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MTL-2	1
2	Cd(II), 1	2
3	Cd(II), 2	2
4	Cd(II), 3	2

Entities:

Entity 1, MTL-2 63 residues - Formula weight is not available

1

MET

VAL

CYS

LYS

CYS

ASP

CYS

LYS

ASN

GLN

2

ASN

CYS

SER

CYS

ASN

THR

GLY

THR

LYS

ASP

3

CYS

ASP

CYS

SER

ASP

ALA

LYS

CYS

GLU

4

GLN

TYR

CYS

PRO

THR

ALA

SER

GLU

LYS

5

LYS

CYS

LYS

SER

GLY

CYS

ALA

GLY

6

CYS

LYS

CYS

ALA

ASN

CYS

GLU

CYS

ALA

GLN

7

ALA

HIS

Entity 2, Cd(II), 1 - Cd - 112.411 Da.

1

CD

Samples:

sample_1: Cd6MTL2 1 mM; TRIS, [U-2H], 20 mM; sodium chloride 50 mM

sample_2: Cd6MTL2 1 mM; TRIS, [U-2H], 50 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.12 M; pH: 7.0; pressure: 1 atm; temperature: 303 K

sample_conditions_2: ionic strength: 0.06 M; pH: 298; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-111Cd HSQC	sample_1	isotropic	sample_conditions_2

Software:

CYANA v2.1 - structure solution

TOPSPIN v2.1 - collection

SPARKY v3.114 - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz
Bruker DRX 500 MHz