BMRB Entry 51534

Title:
Sox2-Nterminal(aa1-42)
Deposition date:
2022-07-26
Original release date:
2022-08-26
Authors:
Theillet, Francois-Xavier
Citation:

Citation: Theillet, Francois-Xavier. "Advanced NMR characterization and sensitive detection of isoAs-partate in proteins."  .

Assembly members:

Assembly members:
entity_1, polymer, 43 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet41b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GMYNMMETELKPPGPQQTSG GGGGNSTAAAAGGNQKNSPD RVK

Data sets:
Data typeCount
13C chemical shifts115
15N chemical shifts37
1H chemical shifts37

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sox2(aa1-42)1

Entities:

Entity 1, Sox2(aa1-42) 43 residues - Formula weight is not available

One supplementary Glycine in N-terminal, remaining from Tev-motif (ENLYFQG) after cleavage by Tev-protease.

1   GLYMETTYRASNMETMETGLUTHRGLULEU
2   LYSPROPROGLYPROGLNGLNTHRSERGLY
3   GLYGLYGLYGLYASNSERTHRALAALAALA
4   ALAGLYGLYASNGLNLYSASNSERPROASP
5   ARGVALLYS

Samples:

sample_1: Sox2(aa1-42), [U-98% 13C; U-98% 15N], 0.8 ± 0.1 mM; HEPES 10 mM; sodium chloride 50 mM; DSS 0.5 ± 0.1 mM; protease inhibitor cocktail 2 tablet/100mL; GST-His6-Tev, [U-98% 13C; U-98% 15N], 0.8 ± 0.1 mM; DTT 2 mM; TCEP 5 mM

sample_conditions_1: ionic strength: 0.06 M; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CA)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.1 - collection, processing

CcpNMR v2.4 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks