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BMRB Entry 51445 BMRB - Biological Magnetic Resonance Bank

BMRB Entry 51445

Title:
Chemical shift assignments for alanine and valine methyls of Robo1-Ig1-2-LnLoop
Deposition date:
2022-05-16
Original release date:
2022-06-14
Authors:
Williams, Robert; Huang, Chin; Moremen, Kelley; Amster, I.; Prestegard, James
Citation:

Citation: Williams, Robert; Huang, Chin; Moremen, Kelley; Amster, I.; Prestegard, James. "NMR analysis suggests the terminal domains of Robo1 remain extended but are rigidified in the presence of heparan sulfate"  Sci. Rep. 12, 14769-14769 (2022).
PubMed: 36042257

Assembly members:

Assembly members:
entity_1, polymer, 222 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: mammalian HEK   Vector: pGEn2

Data sets:
Data typeCount
13C chemical shifts29
1H chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Robo1-Ig1-2-LnLoop1

Entities:

Entity 1, Robo1-Ig1-2-LnLoop 222 residues - Formula weight is not available

Numbering is consistent with uniprot Q9YN67 from 58-221. Then a 16 residue lanthanide binding tag replaces residues 222 and 223, resulting in the following sequence numbers differing from Q9YN67 by +14.

1   GLYSERARGLEUARGGLNGLUASPPHEPRO
2   PROARGILEVALGLUHISPROSERASPLEU
3   ILEVALSERLYSGLYGLUPROALATHRLEU
4   ASNCYSLYSALAGLUGLYARGPROTHRPRO
5   THRILEGLUTRPTYRLYSGLYGLYGLUARG
6   VALGLUTHRASPLYSASPASPPROARGSER
7   HISARGMETLEULEUPROSERGLYSERLEU
8   PHEPHELEUARGILEVALHISGLYARGLYS
9   SERARGPROASPGLUGLYVALTYRVALCYS
10   VALALAARGASNTYRLEUGLYGLUALAVAL
11   SERHISASNALASERLEUGLUVALALAILE
12   LEUARGASPASPPHEARGGLNASNPROSER
13   ASPVALMETVALALAVALGLYGLUPROALA
14   VALMETGLUCYSGLNPROPROARGGLYHIS
15   PROGLUPROTHRILESERTRPLYSLYSASP
16   GLYSERPROLEUASPASPLYSASPGLUARG
17   ILETHRILEARGCYSALAASPTHRASNASN
18   ASPGLYALATYRGLUGLYASPGLULEUCYS
19   LYSLEUMETILETHRTYRTHRARGLYSSER
20   ASPALAGLYLYSTYRVALCYSVALGLYTHR
21   ASNMETVALGLYGLUARGGLUSERGLUVAL
22   ALAGLULEUTHRVALLEUGLUARGPROSER
23   PHEVAL

Samples:

sample_1: Robo1-Ig1-2-LnLoop, [U-13C; 49% HB-Ala; 99% HG-Val], 300 uM; D2O, [U-99% 2H], 10%; TRIS 25 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-detected HSQCsample_1isotropicsample_conditions_1
3D 13C-detected NOESYsample_1isotropicsample_conditions_1
2D 13C-detected TOCSYsample_1isotropicsample_conditions_1

Software:

ASSIGN_SLPGUI v2.4.6 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 900 MHz