BMRB Entry 51265

Title:
solution structure of conotoxin mr1.7
Deposition date:
2022-01-11
Original release date:
2022-01-17
Authors:
Zhao, Cong; Du, Weihong
Citation:

Citation: Zhao, Cong; Du, Weihong; Dai, Qiuyun. "Comparison of the solution structures of two -conotoxins with different intercysteine loop pattern"  .

Assembly members:

Assembly members:
entity_1, polymer, 17 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Conus vitulinus   Taxonomy ID: 42752   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus vitulinus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: PECCTHPACHVSHPELC

Data sets:
Data typeCount
1H chemical shifts102

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1conotoxin Mr1.71

Entities:

Entity 1, conotoxin Mr1.7 17 residues - Formula weight is not available

1   PROGLUCYSCYSTHRHISPROALACYSHIS
2   VALSERHISPROGLULEUCYS

Samples:

sample_1: conotoxin Mr1.7 4 mM; t-butanol 0.01%

sample_2: conotoxin Mr1.7 4 mM; t-butanol 0.01%

sample_conditions_1: pH: 3.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v2.1 - processing

CYANA v2.1 - geometry optimization, structure solution

SPARKY v3.114 - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 400 MHz