BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5126

Title: Complete 1H, 15N and 13C Assignment of the Carboxyl Terminal Domain of the Ciliary Neurotrophic Factor Receptor (CNTFR)   PubMed: 11885987

Deposition date: 2001-08-28 Original release date: 2002-04-08

Authors: Man, D.; Xia, Y.; Sze, K.; Smith, D.; He, W.; Ip, N.; Zhu, G.

Citation: Man, D.; Xia, Y.; Sze, K.; Smith, D.; He, W.; Ip, N.; Zhu, G.. "Complete 1H, 15N and 13C Assignment of the Carboxyl Terminal Domain of the Ciliary Neurotrophic Factor Receptor (CNTFR)"  J. Biomol. NMR 22, 95-96 (2002).

Assembly members:
Ciliary Neurotrophic Factor Receptor, polymer, 109 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Ciliary Neurotrophic Factor Receptor: GPLGSVKPDPPENVVARPVP SNPRRLEVTWQTPSTWPDPE SFPLKFFLRYRPLILDQWQH VELSNGTAHTITDAYAGKEY IIQVAAKDNEIGTWSDWSVA AHATPWTEE

Data sets:
Data typeCount
1H chemical shifts730
13C chemical shifts451
15N chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CNTFR subunit 11

Entities:

Entity 1, CNTFR subunit 1 109 residues - Formula weight is not available

1   GLYPROLEUGLYSERVALLYSPROASPPRO
2   PROGLUASNVALVALALAARGPROVALPRO
3   SERASNPROARGARGLEUGLUVALTHRTRP
4   GLNTHRPROSERTHRTRPPROASPPROGLU
5   SERPHEPROLEULYSPHEPHELEUARGTYR
6   ARGPROLEUILELEUASPGLNTRPGLNHIS
7   VALGLULEUSERASNGLYTHRALAHISTHR
8   ILETHRASPALATYRALAGLYLYSGLUTYR
9   ILEILEGLNVALALAALALYSASPASNGLU
10   ILEGLYTHRTRPSERASPTRPSERVALALA
11   ALAHISALATHRPROTRPTHRGLUGLU

Samples:

sample_1: Ciliary Neurotrophic Factor Receptor, [U-99% 15N], 2 mM

sample_2: Ciliary Neurotrophic Factor Receptor, [U-99% 13C; U-99% 15N], 1.3 mM

Ex-cond_1: pH*: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HACANnot availablenot availablenot available
HACA(CO)Nnot availablenot availablenot available
HACACOnot availablenot availablenot available
HNCOnot availablenot availablenot available
3D 15N separated TOCSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCC-TOCSY-NNHnot availablenot availablenot available

Software:

nmrpipe v1.6 - processing, assignment

pipp - processing, assignment

sparky v2.9 - processing, assignment

NMR spectrometers:

  • Varian inova 750 MHz
  • Varian inova 500 MHz

Related Database Links:

PDB
DBJ BAE23207 BAG54504 BAI44631 BAI46662
GB AAA35707 AAA91337 AAB25290 AAB25918 AAC25711
REF NP_001003353 NP_001003929 NP_001129528 NP_001193940 NP_001244612
SP O88507 P26992 Q08406 Q71DR4
TPG DAA26659

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts