BMRB Entry 51226

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51226

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Title:
Backbone and side-chain chemical shift assignment of mycobacterial antitoxin MazE6 from MazEF6 TA system.
Deposition date:
2021-12-10
Original release date:
2022-09-20
Authors:
Kumari, Khushboo; Sarma, Siddhartha
Citation:

Citation: Kumari, Khushboo; Sarma, Siddhartha. "Structural and mutational analysis of MazE6-operator DNA complex provide insights into autoregulation of toxin-antitoxin systems"  Commun. Biol. 5, 963-963 (2022).
PubMed: 36109664

Assembly members:

Assembly members:
entity_1, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKTAISLPDETFDRVSRRAS ELGMSRSEFFTKAAQRYLHE LDAQLLTGQIDRALESIHGT DEAEALAVANAYRVLETMDD EW

Data sets:
Data typeCount
13C chemical shifts291
15N chemical shifts80
1H chemical shifts331

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Chain A1
2Chain B1

Entities:

Entity 1, Chain A 82 residues - Formula weight is not available

1   METLYSTHRALAILESERLEUPROASPGLU
2   THRPHEASPARGVALSERARGARGALASER
3   GLULEUGLYMETSERARGSERGLUPHEPHE
4   THRLYSALAALAGLNARGTYRLEUHISGLU
5   LEUASPALAGLNLEULEUTHRGLYGLNILE
6   ASPARGALALEUGLUSERILEHISGLYTHR
7   ASPGLUALAGLUALALEUALAVALALAASN
8   ALATYRARGVALLEUGLUTHRMETASPASP
9   GLUTRP

Samples:

sample_1: MazE6 Homodimer 300 uM; potassium phosphate 20 mM; sodium chloride 50 mM; EDTA 1 mM; sodium azide 0.01%; DSS 0.5 mM; D2O, [U-99% 2H], 10%

sample_2: MazE6 Homodimer, [U-99% 15N], 400 uM; potassium phosphate 20 mM; sodium chloride 50 mM; EDTA 1 mM; sodium azide 0.01%; DSS 0.5 mM; D2O, [U-99% 2H], 10%

sample_3: MazE6 Homodimer, [U-99% 13C; U-99% 15N], 400 uM; potassium phosphate 20 mM; sodium chloride 50 mM; EDTA 1 mM; sodium azide 0.01%; DSS 0.5 mM; D2O, [U-99% 2H], 10%

sample_4: MazE6 Homodimer, [U-13C; U-15N; U-2H; 99% 1HD-Ile,Leu; 99% 1HG-Val], 400 uM; potassium phosphate 20 mM; sodium chloride 50 mM; EDTA 1 mM; sodium azide 0.01%; DSS 0.5 mM; D2O, [U-99% 2H], 10%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCACBsample_4isotropicsample_conditions_1
3D HN(CO)CACBsample_4isotropicsample_conditions_1
3D HNCOsample_4isotropicsample_conditions_1
3D HN(CA)COsample_4isotropicsample_conditions_1
3D C(CO)NHsample_4isotropicsample_conditions_1
3D 15N - edited NOESYsample_4isotropicsample_conditions_1
3D H(CCO)NHsample_4isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D 1H-15N TOCSYsample_3isotropicsample_conditions_1

Software:

VNMRj v4.2 - Data acquisition

NMRPipe - Data processing

CcpNMR - data analysis

NMR spectrometers:

  • Agilent DDS2 14.1 T MHz

Related Database Links:

NCBI 14515890

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks