BMRB Entry 51223

Title:
1H15N HSQC chemical shifts and Relaxation Parameters for Y99C MIF Homotrimers
Deposition date:
2021-12-07
Original release date:
2022-03-09
Authors:
Skeens, Erin; Lisi, George
Citation:

Citation: Skeens, Erin; Pantouris, Georgios; Shah, Dilip; Manjula, Ramu; Ombrello, Michael; Maluf, N.; Bhandari, Vineet; Lisi, George; Lolis, Elias. "A Cysteine Variant at an Allosteric Site Alters MIF Dynamics and Biological Function in Homo- and Heterotrimeric Assemblies"  Front. Mol. Biosci. 9, 783669-783669 (2022).
PubMed: 35252348

Assembly members:

Assembly members:
entity_1, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a

Data sets:
Data typeCount
15N chemical shifts94
1H chemical shifts94
T1 relaxation values83
T2 relaxation values81
heteronuclear NOE values82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Y99C MIF chain 11
2Y99C MIF chain 21
3Y99C MIF chain 31

Entities:

Entity 1, Y99C MIF chain 1 114 residues - Formula weight is not available

1   PROMETPHEILEVALASNTHRASNVALPRO
2   ARGALASERVALPROASPGLYPHELEUSER
3   GLULEUTHRGLNGLNLEUALAGLNALATHR
4   GLYLYSPROPROGLNTYRILEALAVALHIS
5   VALVALPROASPGLNLEUMETALAPHEGLY
6   GLYSERSERGLUPROCYSALALEUCYSSER
7   LEUHISSERILEGLYLYSILEGLYGLYALA
8   GLNASNARGSERTYRSERLYSLEULEUCYS
9   GLYLEULEUALAGLUARGLEUARGILESER
10   PROASPARGVALTYRILEASNTYRCYSASP
11   METASNALAALAASNVALGLYTRPASNASN
12   SERTHRPHEALA

Samples:

sample_1: Y99C MIF trimers, [U-99% 15N], 1.0 mM; sodium phosphate 20 mM; EDTA 1.0 mM; D2O 10%

sample_conditions_1: ionic strength: 20.0 mM; pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
T1/R1 relaxationsample_1isotropicsample_conditions_1
T2/R2 relaxationsample_1isotropicsample_conditions_1
1H-15N heteronoesample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

NMRDraw - processing

NMRFAM-SPARKY - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks