BMRB Entry 51076

Title:
Methyl side-chain chemical shift assignments for ribosomal protein bL12
Deposition date:
2021-09-06
Original release date:
2021-09-12
Authors:
Burridge, Charles; Waudby, Christopher; Wlodarski, Tomasz; Cassaignau, AnaIs; Cabrita, Lisa; Christodoulou, John
Citation:

Citation: Burridge, Charles; Waudby, Christopher; Wlodarski, Tomasz; Cassaignau, AnaIs; Cabrita, Lisa; Christodoulou, John. "Nascent chain dynamics and ribosome interactions within folded ribosome-nascent chain complexes observed by NMR spectroscopy"  Chem. Sci. 12, 13120-13126 (2021).
PubMed: 34745542

Assembly members:

Assembly members:
entity_1, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b(+)

Data sets:
Data typeCount
13C chemical shifts14
1H chemical shifts42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bL121

Entities:

Entity 1, bL12 121 residues - Formula weight is not available

E.coli ribosomal protein bL12

1   METSERILETHRLYSASPGLNILEILEGLU
2   ALAVALALAALAMETSERVALMETASPVAL
3   VALGLULEUILESERALAMETGLUGLULYS
4   PHEGLYVALSERALAALAALAALAVALALA
5   VALALAALAGLYPROVALGLUALAALAGLU
6   GLULYSTHRGLUPHEASPVALILELEULYS
7   ALAALAGLYALAASNLYSVALALAVALILE
8   LYSALAVALARGGLYALATHRGLYLEUGLY
9   LEULYSGLUALALYSASPLEUVALGLUSER
10   ALAPROALAALALEULYSGLUGLYVALSER
11   LYSASPASPALAGLUALALEULYSLYSALA
12   LEUGLUGLUALAGLYALAGLUVALGLUVAL
13   LYS

Samples:

sample_1: bL12, [U-13C; U-15N], 300 uM; HEPES 10 mM; magnesium chloride 12 mM; ammonium chloride 30 mM; BME 2 mM; DSS 0.001%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
4D HCccoNH TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HMQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

MATLAB - chemical shift assignment

NMRFAM-SPARKY v1.414 - chemical shift assignment

TOPSPIN - collection

NMRPipe - processing

SMILE - processing

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Related Database Links:

UNP P0A7K2
AlphaFold Q2M8S2