BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5106

Title: An NMR Approach to Structural Proteomics   PubMed: 11854485

Authors: Yee, Adelinda; Chang, Xiaoqing; Pineda-Lucena, Antonio; Wu, Bin; Semesi, Anthony; Le, Brian; Ramelot, Theresa; Lee, Gregory; Bhattacharyya, Sudeepa; Gutierrez, Pablo; Denisov, Aleksej; Lee, Chang-Hun; Cort, John; Kozlov, Guennadi; Liao, Jack; Finak, Grzegorz; Chen, Limin; Wishart, David; Lee, Weontae; McIntosh, Lawrence; Gehring, Kalle; Kennedy, Michael; Edwards, Aled; Arrowsmith, Cheryl

Citation: Yee, Adelinda; Chang, Xiaoqing; Pineda-Lucena, Antonio; Wu, Bin; Semesi, Anthony; Le, Brian; Ramelot, Theresa; Lee, Gregory; Bhattacharyya, Sudeepa; Gutierrez, Pablo; Denisov, Aleksej; Lee, Chang-Hun; Cort, John; Kozlov, Guennadi; Liao, Jack; Finak, Grzegorz; Chen, Limin; Wishart, David; Lee, Weontae; McIntosh, Lawrence; Gehring, Kalle; Kennedy, Michael; Edwards, Aled; Arrowsmith, Cheryl. "An NMR Approach to Structural Proteomics"  Proc. Natl. Acad. Sci. U.S.A. 99, 1825-1830 (2002).

Assembly members:
MTH1743, polymer, 70 residues, Formula weight is not available

Natural source:   Common Name: Methanothermobacter thermoautotrophicus   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Methanobacterium thermoautotrophicum

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
MTH1743: MVIGMKFTVITDDGKKILES GAPRRIKDVLGELEIPIETV VVKKNGQIVIDEEEIFDGDI IEVIRVIYGG

Data sets:
Data typeCount
1H chemical shifts524
13C chemical shifts305
15N chemical shifts68

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MTH17431

Entities:

Entity 1, MTH1743 70 residues - Formula weight is not available

1   METVALILEGLYMETLYSPHETHRVALILE
2   THRASPASPGLYLYSLYSILELEUGLUSER
3   GLYALAPROARGARGILELYSASPVALLEU
4   GLYGLULEUGLUILEPROILEGLUTHRVAL
5   VALVALLYSLYSASNGLYGLNILEVALILE
6   ASPGLUGLUGLUILEPHEASPGLYASPILE
7   ILEGLUVALILEARGVALILETYRGLYGLY

Samples:

SAMPLE_1: MTH1743, [U-13C; U-15N], mM

EX-COND_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
CCC-TOCSY-NHnot availablenot availablenot available
3D CN Simultaneous NOESYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available
HCC-TOCSY-NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • VARIAN INOVA 500 MHz
  • VARIAN INOVA 600 MHz

Related Database Links:

PDB
DBJ BAM70842
GB AAB86213
REF NP_276853 WP_010877349