BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5093

Title: Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments for Ribosome-binding Factor A (RbfA)   PubMed: 11824762

Authors: Swapna, G.; Shukla, Kamal; Huang, YJ; Ke, H.; Xia, B.; Inouye, Masayori; Montelione, Gaetano

Citation: Swapna, G.; Shukla, Kamal; Huang, YJ; Ke, H.; Xia, B.; Inouye, Masayori; Montelione, Gaetano. "Resonance assignments for cold-shock protein ribosome-binding factor A (RbfA) from Escherichia coli"  J. Biomol. NMR 21, 389-390 (2001).

Assembly members:
RbfA, polymer, 108 residues, 12300 Da.

Natural source:   Common Name: E. coli.   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RbfA: MAKEFGRPQRVAQEMQKEIA LILQREIKDPRLGMMTTVSG VEMSRDLAYAKVYVTFLNDK DEDAVKAGIKALQEASGFIR SLLGKAMRLRIVPELTFFYD NSLVEGMR

Data sets:
Data typeCount
1H chemical shifts727
13C chemical shifts442
15N chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ribosome-binding Factor A1

Entities:

Entity 1, Ribosome-binding Factor A 108 residues - 12300 Da.

1   METALALYSGLUPHEGLYARGPROGLNARG
2   VALALAGLNGLUMETGLNLYSGLUILEALA
3   LEUILELEUGLNARGGLUILELYSASPPRO
4   ARGLEUGLYMETMETTHRTHRVALSERGLY
5   VALGLUMETSERARGASPLEUALATYRALA
6   LYSVALTYRVALTHRPHELEUASNASPLYS
7   ASPGLUASPALAVALLYSALAGLYILELYS
8   ALALEUGLNGLUALASERGLYPHEILEARG
9   SERLEULEUGLYLYSALAMETARGLEUARG
10   ILEVALPROGLULEUTHRPHEPHETYRASP
11   ASNSERLEUVALGLUGLYMETARG

Samples:

sample_1: RbfA, [U-13C; U-15N], 1.6 mM

condition_1: pH: 5.05; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
(HA)CA(CO)NHnot availablenot availablenot available
HA(CA)(CO)NHnot availablenot availablenot available
(HA)CANHnot availablenot availablenot available
HA(CA)NHnot availablenot availablenot available
H(CCCO)NH-TOCSYnot availablenot availablenot available
(H)CC(CO)NH-TOCSYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
13C-edited NOESYnot availablenot availablenot available
15N-edited NOESYnot availablenot availablenot available

Software:

VNMR v6.3b - data processing

SPARKY v3.91 - peak picking

AUTOASSIGN v1.7.8 - automated backbone 1H, 13C and 15N assignments

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 18147
PDB
DBJ BAB37471 BAE77213 BAG78977 BAH65352 BAI27447
EMBL CAA31634 CAA32020 CAP77629 CAQ33502 CAQ90639
GB AAA57970 AAC76201 AAG58303 AAL22157 AAN44675
REF NP_289743 NP_312075 NP_417636 NP_462198 NP_708968
SP A1AG72 A6TEI6 A7ZS64 A8A4Y3 A8AQ56