BMRB Entry 50847

Title:
ZEBRA-responsive element 2 DNA
Deposition date:
2021-03-25
Original release date:
2021-11-24
Authors:
Gunther, Johannes
Citation:

Citation: Bernaudat, Florent; Gustems, Montse; Gunther, Johannes; Oliva, Mizar; Buschle, Alexander; Gobel, Christine; Pagniez, Priscilla; Lupo, Julien; Signor, Luca; Muller, Christoph; Morand, Patrice; Sattler, Michael; Hammerschmidt, Wolfgang; Petosa, Carlo. "Structural basis of DNA methylation-dependent site selectivity of the Epstein-Barr virus lytic switch protein ZEBRA/Zta/BZLF1"  Nucleic Acids Res. 50, 490-511 (2022).
PubMed: 34893887

Assembly members:

Assembly members:
entity_1, polymer, 18 residues, Formula weight is not available
entity_2, polymer, 18 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human gammaherpesvirus 4   Taxonomy ID: 10376   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lymphocryptovirus Epstein Barr Virus

Experimental source:

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GCTTATGAGCGATTTTAT
entity_2: CGAATACTCGCTAAAATA

Data sets:
Data typeCount
1H chemical shifts42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Chain A1
2Chain B2

Entities:

Entity 1, Chain A 18 residues - Formula weight is not available

1   DGDCDTDTDADTDGDADGDC
2   DGDADTDTDTDTDADT

Entity 2, Chain B 18 residues - Formula weight is not available

1   DCDGDADADTDADCDTDCDG
2   DCDTDADADADADTDA

Samples:

sample_1: ZEBRA-responsive element 2 DNA (ZRE2) 400 uM; sodium phosphate 50 mM; sodium chloride 100 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment and data analysis

TOPSPIN v3.5 pl6 - processing

NMR spectrometers:

  • Bruker AVANCE III HD 950 MHz