BMRB Entry 5081

Title:
1H, 15N and 13C resonance assignments for the Gallium protoporphyrin IX-HasAsm hemophore complex
Deposition date:
2001-07-16
Original release date:
2001-11-07
Authors:
Deniau, Clarisse; Couprie, Joel; Simenel, Catherine; Kumar, Vera; Stojiljkovic, Igor; Wandersman, Cecile; Delepierre, Muriel; Lecroisey, Anne
Citation:

Citation: Deniau, Clarisse; Couprie, Joel; Simenel, Catherine; Kumar, Vera; Stojiljkovic, Igor; Wandersman, Cecile; Delepierre, Muriel; Lecroisey, Anne. "Letter to the Editor: 1H, 15N and 13C resonance assignments for the Gallium protoporphyrin IX-HasAsm hemophore complex"  J. Biomol. NMR 21, 189-190 (2001).

Assembly members:

Assembly members:
HasAsm, polymer, 178 residues, 18915 Da.
GIX, non-polymer, 630.365 Da.

Natural source:

Natural source:   Common Name: Serratia marcescens   Taxonomy ID: 615   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Serratia marcescens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSYC34

Data sets:
Data typeCount
1H chemical shifts375
13C chemical shifts504
15N chemical shifts173

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GaPPIX2
2HasAsm1

Entities:

Entity 2, GaPPIX - C34 H32 Ga N4 O4 - 630.365 Da.

1   GIX

Entity 1, HasAsm 178 residues - 18915 Da.

1   ALAPHESERVALASNTYRASPSERSERPHE
2   GLYGLYTYRSERILEHISASPTYRLEUGLY
3   GLNTRPALASERTHRPHEGLYASPVALASN
4   HISTHRASNGLYASNVALTHRASPALAASN
5   SERGLYGLYPHETYRGLYGLYSERLEUSER
6   GLYSERGLNTYRALAILESERSERTHRALA
7   ASNGLNVALTHRALAPHEVALALAGLYGLY
8   ASNLEUTHRTYRTHRLEUPHEASNGLUPRO
9   ALAHISTHRLEUTYRGLYGLNLEUASPSER
10   LEUSERPHEGLYASPGLYLEUSERGLYGLY
11   ASPTHRSERPROTYRSERILEGLNVALPRO
12   ASPVALSERPHEGLYGLYLEUASNLEUSER
13   SERLEUGLNALAGLNGLYHISASPGLYVAL
14   VALHISGLNVALVALTYRGLYLEUMETSER
15   GLYASPTHRGLYALALEUGLUTHRALALEU
16   ASNGLYILELEUASPASPTYRGLYLEUSER
17   VALASNSERTHRPHEASPGLNVALALAALA
18   ALATHRALAVALGLYVALGLNHIS

Samples:

sample_1: HasAsm, [U-95% 13C; U-95% 15N], 2.0 mM; protoporphyrin IX containing Gallium 2.0 mM; Na2HPO4 20 mM; NaH2PO4 20 mM

Ex-cond: pH: 5.6; temperature: 303 K; ionic strength: 0.02 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableEx-cond
HNCOsample_1not availableEx-cond
HNCACBsample_1not availableEx-cond
CBCA(CO)NHsample_1not availableEx-cond

Software:

NMRpipe - processing

XEASY v1.2 - analysis

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

SWISS-PROT Q54450
EMBL CAA57068
PDB
BMRB 4309

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks