BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5080

Title: Backbone dynamics of the functional domain of Paracoccus denitrificans Cytochrome c552 in the Oxidized state   PubMed: 11591150

Authors: Reincke, Britta; Perez, Carlos; Pristovsek, Primoz; Luecke, Christian; Ludwig, Christian; Loehr, Frank; Rogov, Vladimir; Ludwig, Bernd; Rueterjans, Heinz

Citation: Reincke, Britta; Perez, Carlos; Pristovsek, Primoz; Luecke, Christian; Ludwig, Christian; Loehr, Frank; Rogov, Vladimir; Ludwig, Bernd; Rueterjans, Heinz. "Solution Structure and Dynamics of the Functional Domain of Paracoccus denitrificans Cytochrome c552 in both Redox States "  Biochemistry 40, 12312-12320 (2001).

Assembly members:
Paracoccus denitrificans cytochrome c552, soluble fragment, polymer, 100 residues, 10528 Da.
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: not available   Taxonomy ID: 266   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Paracoccus denitrificans

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Paracoccus denitrificans cytochrome c552, soluble fragment: MADPAAGEKVFGKCKACHKL DGNDGVGPHLNGVVGRTVAG VDGFNYSDPMKAHGGDWTPE ALQEFLTNPKAVVKGTKMAF AGLPKIEDRANLIAYLEGQQ

Data sets:
Data typeCount
T1 relaxation values176
T2 relaxation values176

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cyt c5521
2HEC_ox2

Entities:

Entity 1, cyt c552 100 residues - 10528 Da.

1   METALAASPPROALAALAGLYGLULYSVAL
2   PHEGLYLYSCYSLYSALACYSHISLYSLEU
3   ASPGLYASNASPGLYVALGLYPROHISLEU
4   ASNGLYVALVALGLYARGTHRVALALAGLY
5   VALASPGLYPHEASNTYRSERASPPROMET
6   LYSALAHISGLYGLYASPTRPTHRPROGLU
7   ALALEUGLNGLUPHELEUTHRASNPROLYS
8   ALAVALVALLYSGLYTHRLYSMETALAPHE
9   ALAGLYLEUPROLYSILEGLUASPARGALA
10   ASNLEUILEALATYRLEUGLUGLYGLNGLN

Entity 2, HEC_ox - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: Paracoccus denitrificans cytochrome c552, soluble fragment, [U-96% 15N], 1.5 – 4 mM; PROTOPORPHYRIN IX CONTAINING FE1.5 – 4 mM; potassium phosphate 20 mM; D2O 5%; H2O 95%

cond_set_1: pH: 5.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availablecond_set_1

Software:

XWIN-NMR v1.3, Bruker - data collection and processing

RMX vrev. II/1997 - data analysis

MODELFREE v4.01 - data analysis

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz

Related Database Links:

BMRB 4471 4777 5079
PDB
EMBL CAA49830
GB ABL69905
REF WP_011748102 YP_915601
SP P54820