BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5070

Title: Structure and dynamics of the anticodon-arm binding domain of Bacillus stearothermophilus tyrosyl-tRNA synthetase

Authors: Guijarro, J.; Pintar, Alessandro; Prochnicka-Chalufour, Ada; Guez, Valerie; Gilquin, Bernard; Bedouelle, Hugues; Delepierre, Muriel

Citation: Guijarro, J.; Pintar, Alessandro; Prochnicka-Chalufour, Ada; Guez, Valerie; Gilquin, Bernard; Bedouelle, Hugues; Delepierre, Muriel. "Structure and dynamics of the anticodon-arm binding domain of Bacillus stearothermophilus tyrosyl-tRNA synthetase"  Structure 10, 311-317 (2002).

Assembly members:
TyrRS C-terminal domain, polymer, 107 residues, 11980.57 Da.

Natural source:   Common Name: not available   Taxonomy ID: 1422   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus stearothermophilus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TyrRS C-terminal domain: ALFSGDIANLTAAEIEQGFK DVPSFVHEGGDVPLVELLVS AGISPSKRQAREDIQNGAIY VNGERLQDVGAILTAEHRLE GRFTVIRRGKKKYYLIRYAL EHHHHHH

Data sets:
Data typeCount
1H chemical shifts666
13C chemical shifts368
15N chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TyrRS C-ter. domain1

Entities:

Entity 1, TyrRS C-ter. domain 107 residues - 11980.57 Da.

1   ALALEUPHESERGLYASPILEALAASNLEU
2   THRALAALAGLUILEGLUGLNGLYPHELYS
3   ASPVALPROSERPHEVALHISGLUGLYGLY
4   ASPVALPROLEUVALGLULEULEUVALSER
5   ALAGLYILESERPROSERLYSARGGLNALA
6   ARGGLUASPILEGLNASNGLYALAILETYR
7   VALASNGLYGLUARGLEUGLNASPVALGLY
8   ALAILELEUTHRALAGLUHISARGLEUGLU
9   GLYARGPHETHRVALILEARGARGGLYLYS
10   LYSLYSTYRTYRLEUILEARGTYRALALEU
11   GLUHISHISHISHISHISHIS

Samples:

sample_1: TyrRS C-terminal domain, [U-15N], 1.2 mM; potassium phosphate 20 mM; H2O 90%; D2O 10%

sample_2: TyrRS C-terminal domain, [U-13C; U-15N], 0.8 mM; potassium phosphate 20 mM; H2O 90%; D2O 10%

Cond_set_1: pH: 6.8; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
15N-edited NOESY-HSQCnot availablenot availablenot available
TOCSY-HSQCnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
H(CC-TOCSY)NNHnot availablenot availablenot available
C(CC-TOCSY)NNHnot availablenot availablenot available
13C-edited HCCH-TOCSYnot availablenot availablenot available

Software:

VNMR v6.1B - acquisition, data processing

XEASY v1.2 - Assignment

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB 1JH3 2TS1 3TS1
DBJ BAD77088 GAD11882 GAJ57796
GB AAA22877 ACX77385 ADI25737 ADU95213 AEV20456
REF WP_011232277 WP_013144460 WP_013524353 WP_014196563 WP_015375638
SP P00952 P04077 Q5KW48