BMRB Entry 5058

Title:
Letter to the Editor: Sequence specific 1H, 13C and 15N resonance assignments of human GABA receptor associated protein
Deposition date:
2001-06-14
Original release date:
2001-11-06
Authors:
Stangler, Thomas; Mayr, Lorenz; Dingley, Andrew; Luge, Cornelia; Willbold, Dieter
Citation:

Citation: Stangler, Thomas; Mayr, Lorenz; Dingley, Andrew; Luge, Cornelia; Willbold, Dieter. "Letter to the Editor: Sequence specific 1H, 13C and 15N resonance assignments of human GABA receptor associated protein"  J. Biomol. NMR 21, 183-184 (2001).

Assembly members:

Assembly members:
GABARAP, polymer, 119 residues, 14077 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pGEX-4T-1

Data sets:
Data typeCount
1H chemical shifts844
13C chemical shifts527
15N chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GABARAP1

Entities:

Entity 1, GABARAP 119 residues - 14077 Da.

1   GLYSERMETLYSPHEVALTYRLYSGLUGLU
2   HISPROPHEGLULYSARGARGSERGLUGLY
3   GLULYSILEARGLYSLYSTYRPROASPARG
4   VALPROVALILEVALGLULYSALAPROLYS
5   ALAARGILEGLYASPLEUASPLYSLYSLYS
6   TYRLEUVALPROSERASPLEUTHRVALGLY
7   GLNPHETYRPHELEUILEARGLYSARGILE
8   HISLEUARGALAGLUASPALALEUPHEPHE
9   PHEVALASNASNVALILEPROPROTHRSER
10   ALATHRMETGLYGLNLEUTYRGLNGLUHIS
11   HISGLUGLUASPPHEPHELEUTYRILEALA
12   TYRSERASPGLUSERVALTYRGLYLEU

Samples:

sample_1: GABARAP, [U-15N; U-13C], 0.8 mM; sodium chloride 100 mM; potassium chloride 100 mM; sodium oxalate 25 mM; sodium azide 0.02%; EDTA 0.05 mM; PMSF 0.1 mM; Glycerol mM

conditions_1: pH: 5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HCCONH-TOCSYnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available

Software:

NMRPipe - data processing

NMRView v5.0.3 - spectral analysis

NMR spectrometers:

  • Varian Unity INOVA 750 MHz
  • Bruker DMX 600 MHz

Related Database Links:

BMRB 5064 5128
PDB
DBJ BAB21549 BAB22426 BAB27806 BAG35138
EMBL CAG00707 CAG33324 CAG47031 CAP17839 CAP17840
GB AAD02337 AAD32455 AAD47641 AAD47642 AAD47643
REF NP_001011192 NP_001013278 NP_001029220 NP_001075611 NP_001084530
SP O95166 P60517 Q8MK68 Q9DCD6 Q9GJW7
TPG DAA18837
AlphaFold Q9DCD6 O95166 P60517 Q8MK68 Q9GJW7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks