BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5032

Title: 1H, 13C and 15N Resonance Assignments of the DNA Binding Domain of the Human Forkhead Transcription Factor AFX   PubMed: 10921784

Authors: Weigelt, Johan; Climent, Isabel; Dahlman-Wright, K.; Wikstrom, Mats

Citation: Weigelt, Johan; Climent, Isabel; Dahlman-Wright, K.; Wikstrom, Mats. "Letter to the Editor: 1H, 13C and 15N Resonance Assignments of the DNA Binding Domain of the Human Forkhead Transcription Factor AFX"  J. Biomol. NMR 17, 181-182 (2000).

Assembly members:
AFX DNA binding domain, polymer, 108 residues, Formula weight is not available
Insulin response element from IGFBP-1 promoter, polymer, 16 residues, Formula weight is not available
Insulin response element from IGFBP-1 promoter, polymer, 16 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
AFX DNA binding domain: MRGSHHHHHHGSRRNAWGNQ SYAELISQAIESAPEKRLTL AQIYEWMVRTVPYFKDKGDS NSSAGWKNSIRHNLSLHSKF IKVHNEATGKSSWWMLNPEG GKSGKAPR
Insulin response element from IGFBP-1 promoter: CAAGCAAAACAAACCA
Insulin response element from IGFBP-1 promoter: TGGTTTGTTTTGCTTG

Data sets:
Data typeCount
1H chemical shifts75
13C chemical shifts147
15N chemical shifts75

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AFX DNA binding domain1
216mer insulin response sequence, strand 12
316mer insulin response sequence, strand 23

Entities:

Entity 1, AFX DNA binding domain 108 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERARGARGASNALATRPGLYASNGLN
3   SERTYRALAGLULEUILESERGLNALAILE
4   GLUSERALAPROGLULYSARGLEUTHRLEU
5   ALAGLNILETYRGLUTRPMETVALARGTHR
6   VALPROTYRPHELYSASPLYSGLYASPSER
7   ASNSERSERALAGLYTRPLYSASNSERILE
8   ARGHISASNLEUSERLEUHISSERLYSPHE
9   ILELYSVALHISASNGLUALATHRGLYLYS
10   SERSERTRPTRPMETLEUASNPROGLUGLY
11   GLYLYSSERGLYLYSALAPROARG

Entity 2, 16mer insulin response sequence, strand 1 16 residues - Formula weight is not available

1   DCDADADGDCDADADADADC
2   DADADADCDCDA

Entity 3, 16mer insulin response sequence, strand 2 16 residues - Formula weight is not available

1   DTDGDGDTDTDTDGDTDTDT
2   DTDGDCDTDTDG

Samples:

sample_1: AFX DNA binding domain, [U-13C; U-15N; U-90% 2H], 0.5 mM; Insulin response element from IGFBP-1 promoter 0.5 mM; Insulin response element from IGFBP-1 promoter 0.5 mM; sodium phospate 20 mM; NaCl 50 mM; MgCl2 2 mM; NaN3 0.02 % w/v

Exp_cond: pH: 6.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N TROSYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
HNCACB-TROSYnot availablenot availablenot available
HNCA-TROSYnot availablenot availablenot available

Software:

nmrPipe - processing

ANSIG - analysis

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA86199 BAE34214 BAG61928 BAG73163
EMBL CAA63819 CAA72156
GB AAD42108 AAH99460 AAI06762 AAI39276 AAI39305
REF NP_001094747 NP_001100413 NP_005929 NP_061259 XP_002720130
SP P98177 Q9WVH3
TPG DAA12928