BMRB Entry 50305

Name: Assignment NaK2K Y66F channel
Published: 2021-06-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50305

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment NaK2K Y66F channel

Deposition date:

2020-06-05

Original release date:

2021-06-15

Authors:

Hendriks, Kitty; Oester, Carl; Shi, Chaowei; Sun, Han; Lange, Adam

Citation:

Citation: Hendriks, Kitty; Oester, Carl; Shi, Chaowei; Sun, Han; Lange, Adam. "Sodium ions do not stabilize the selectivity filter of a potassium channel." J. Mol. Biol. 433, 167091-167091 (2021).
PubMed: 34090923

Assembly members:

Assembly members:
entity_1, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacillus cereus Taxonomy ID: 1396 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus cereus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAKDKEFQVLFVLTILTLIS GTIFYSTVEGLRPIDALYFS VVTLTTVGFGDFSPQTDFGK IFTILYIFIGIGLVFGFIHK LAVNVQLPSILSNLVPRGSR SHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2

Data type	Count
13C chemical shifts	371
15N chemical shifts	103

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NaK1K, chain 1	1
2	NaK1K, chain 2	1
3	NaK1K, chain 3	1
4	NaK1K, chain 4	1

Entities:

Entity 1, NaK1K, chain 1 107 residues - Formula weight is not available

1

MET

ALA

LYS

ASP

LYS

GLU

PHE

GLN

VAL

LEU

2

PHE

VAL

LEU

THR

ILE

LEU

THR

LEU

ILE

SER

3

GLY

THR

ILE

PHE

TYR

SER

THR

VAL

GLU

GLY

4

LEU

ARG

PRO

ILE

ASP

ALA

LEU

TYR

PHE

SER

5

VAL

THR

LEU

THR

VAL

GLY

PHE

GLY

6

ASP

PHE

SER

PRO

GLN

THR

ASP

PHE

GLY

LYS

7

ILE

PHE

THR

ILE

LEU

TYR

ILE

PHE

ILE

GLY

8

ILE

GLY

LEU

VAL

PHE

GLY

PHE

ILE

HIS

LYS

9

LEU

ALA

VAL

ASN

VAL

GLN

LEU

PRO

SER

ILE

10

LEU

SER

ASN

LEU

VAL

PRO

ARG

GLY

SER

ARG

11

SER

HIS

Samples:

sample_1: NaK1K tetramer, [U-98% 13C; U-98% 15N], 4.6 mg; potassium chloride 50 mM; TRIS 20 mM; E. coli total lipid extract 2.3 mg

sample_2: NaK1K tetramer, [U-98% 13C; U-98% 15N], 4.6 mg; sodium chloride 50 mM; TRIS 20 mM; E. coli total lipid extract 2.3 mg

sample_conditions_1: ionic strength: 50 mM; pH: 8; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NCA	sample_1	isotropic	sample_conditions_1
2D NCO	sample_1	isotropic	sample_conditions_1
2D 13C/13C PDSD	sample_1	isotropic	sample_conditions_1
3D NCACO	sample_1	isotropic	sample_conditions_1
3D NCACB	sample_1	isotropic	sample_conditions_1
3D NCOCA	sample_1	isotropic	sample_conditions_1
3D CANCO	sample_1	isotropic	sample_conditions_1
3D NCOCACB	sample_1	isotropic	sample_conditions_1
3D NCACX	sample_1	isotropic	sample_conditions_1
2D NCA	sample_2	isotropic	sample_conditions_1
2D NCO	sample_2	isotropic	sample_conditions_1
2D 13C/13C PDSD	sample_2	isotropic	sample_conditions_1
3D NCACO	sample_2	isotropic	sample_conditions_1
3D NCACB	sample_2	isotropic	sample_conditions_1
3D NCOCA	sample_2	isotropic	sample_conditions_1
3D CANCO	sample_2	isotropic	sample_conditions_1
3D NCOCACB	sample_2	isotropic	sample_conditions_1
3D NCACX	sample_2	isotropic	sample_conditions_1

Software:

CcpNmr Analysis - chemical shift assignment

TOPSPIN - collection, processing

NMR spectrometers:

Bruker Avance 700 MHz