BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50297

Title: ScCM:Apo state, ScCM:YY state, and ScCM:WW state methyl axis order paramaters for Ile and Thr residues.

Deposition date: 2020-05-26 Original release date: 2020-06-02

Authors: Gorman, Scott; Boehr, David

Citation: Gorman, Scott; Winston, Dennis; Sahu, Debashish; Boehr, David. "Different solvent and conformational entropy contributions to the allosteric activation and inhibition mechanisms of yeast chorismate mutase"  Biochemistry ., .-..

Assembly members:
entity_1, polymer, 264 residues, Formula weight is not available

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: MDFTKPETVLNLQNIRDELV RMEDSIIFKFIERSHFATCP SVYEANHPGLEIPNFKGSFL DWALSNLEIAHSRIRRFESP DETPFFPDKIQKSFLPSINY PQILAPYAPEVNYNDKIKKV YIEKIIPLISKRDGDDKNNF GSVATRDIECLQSLSRRIHF GKFVAEAKFQSDIPLYTKLI KSKDVEGIMKNITNSAVEEK ILERLTKKAEVYGVDPTNES GERRITPEYLVKIYKEIVIP ITKEVEVEYLLRRLEESREN LYFQ

Data sets:
Data typeCount
order parameters109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ScCM, Monomer 11
2ScCM, Monomer 21

Entities:

Entity 1, ScCM, Monomer 1 264 residues - Formula weight is not available

The WT sequence ends at "RRLEE". The additional C-terminal residues "SRENLYFQ" are a cloning artifact due to the His tag that is cleaved during purification.

1   METASPPHETHRLYSPROGLUTHRVALLEU
2   ASNLEUGLNASNILEARGASPGLULEUVAL
3   ARGMETGLUASPSERILEILEPHELYSPHE
4   ILEGLUARGSERHISPHEALATHRCYSPRO
5   SERVALTYRGLUALAASNHISPROGLYLEU
6   GLUILEPROASNPHELYSGLYSERPHELEU
7   ASPTRPALALEUSERASNLEUGLUILEALA
8   HISSERARGILEARGARGPHEGLUSERPRO
9   ASPGLUTHRPROPHEPHEPROASPLYSILE
10   GLNLYSSERPHELEUPROSERILEASNTYR
11   PROGLNILELEUALAPROTYRALAPROGLU
12   VALASNTYRASNASPLYSILELYSLYSVAL
13   TYRILEGLULYSILEILEPROLEUILESER
14   LYSARGASPGLYASPASPLYSASNASNPHE
15   GLYSERVALALATHRARGASPILEGLUCYS
16   LEUGLNSERLEUSERARGARGILEHISPHE
17   GLYLYSPHEVALALAGLUALALYSPHEGLN
18   SERASPILEPROLEUTYRTHRLYSLEUILE
19   LYSSERLYSASPVALGLUGLYILEMETLYS
20   ASNILETHRASNSERALAVALGLUGLULYS
21   ILELEUGLUARGLEUTHRLYSLYSALAGLU
22   VALTYRGLYVALASPPROTHRASNGLUSER
23   GLYGLUARGARGILETHRPROGLUTYRLEU
24   VALLYSILETYRLYSGLUILEVALILEPRO
25   ILETHRLYSGLUVALGLUVALGLUTYRLEU
26   LEUARGARGLEUGLUGLUSERARGGLUASN
27   LEUTYRPHEGLN

Samples:

sample_1: ScCM, [U-100% 2H; 100% 1H HD1-Ile; 100% 13C CD1-Ile; 100% 1H HG2-Thr; 100% 13C CG2-Thr], 0.365 mM; potassium phosphate 50 mM; beta-mercaptoethanol 2 mM; sodium azide 0.02%

sample_2: ScCM, [U-100% 2H; 100% 1H HD1-Ile; 100% 13C CD1-Ile; 100% 1H HG2-Thr; 100% 13C CG2-Thr], 0.365 mM; Tyrosine 2.5 mM; potassium phosphate 50 mM; beta-mercaptoethanol 2 mM; sodium azide 0.02%

sample_3: ScCM, [U-100% 2H; 100% 1H HD1-Ile; 100% 13C CD1-Ile; 100% 1H HG2-Thr; 100% 13C CG2-Thr], 0.365 mM; Tryptophan 3 mM; potassium phosphate 50 mM; beta-mercaptoethanol 2 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
Intra-methyl 1H-1H dipolar cross-correlated spin relaxationsample_1isotropicsample_conditions_1
Intra-methyl 1H-1H dipolar cross-correlated spin relaxationsample_2isotropicsample_conditions_1
Intra-methyl 1H-1H dipolar cross-correlated spin relaxationsample_3isotropicsample_conditions_1

Software:

TOPSPIN v3.2 - collection

NMRPipe - data analysis, peak picking, processing

Matlab v2019 - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz MHz

Related Database Links:

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