BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50278

Title: Canonical and modified dsDNA 12mers

Deposition date: 2020-05-17 Original release date: 2020-06-30

Authors: Dubini, Romeo Cosimo Arrigo; Schon, Alexander; Muller, Markus; Carell, Thomas; Rovo, Petra

Citation: Dubini, Romeo Cosimo Arrigo; Schon, Alexander; Muller, Markus; Carell, Thomas; Rovo, Petra. "Impact of 5-Formylcytosine on the Melting Kineticsof DNA by 1H NMR Chemical Exchange"  Not known ., .-..

Assembly members:
Mod12 dsDNA, polymer, 12 residues, Formula weight is not available
Can12 dsDNA, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Mod12 dsDNA: GCGATXGATCGC
Can12 dsDNA: GCGATCGATCGC

Data sets:
Data typeCount
1H chemical shifts159

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mod12 dsDNA1
2Can12 dsDNA2

Entities:

Entity 1, Mod12 dsDNA 12 residues - Formula weight is not available

1   DGDCDGDADT5FCDGDADTDC
2   DGDC

Entity 2, Can12 dsDNA 12 residues - Formula weight is not available

1   DGDCDGDADTDCDGDADTDC
2   DGDC

Samples:

sample_1: Mod12 1.58 mM; D2O 5%; H2O 95%; DSS 25 uM; sodium phosphate 15 mM; sodium azide 0.02%; sodium chloride 25 mM

sample_2: Can12 1.00 mM; D2O 5%; H2O 95%; DSS 25 uM; sodium phosphate 15 mM; sodium azide 0.02%; sodium chloride 25 mM

sample_conditions_1: pH: 7; temperature: 310 K

sample_conditions_2: pH: 7; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
1D 1Hsample_2isotropicsample_conditions_2
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2

Software:

SPARKY v1.414 - chemical shift assignment, peak picking

TOPSPIN v4 - collection, processing

NMR spectrometers:

  • Bruker Avance 800 MHz