BMRB Entry 50260

Title:
1H, 15N and 13C backbone assignments of GDP-bound Rab4a
Deposition date:
2020-05-05
Original release date:
2020-05-07
Authors:
Genier, Samuel; Letourneau, Danny; Parent, Jean-Luc; Lavigne, Pierre
Citation:

Citation: Genier, Samuel; Letourneau, Danny; Gauthier, Esther; Picard, Samuel; Boisvert, Marilou; Parent, Jean-Luc; Lavigne, Pierre. "In-depth NMR characterization of Rab4a structure, nucleotide exchange and hydrolysis kinetics reveals an atypical GTPase profile"  J. Struct. Biol. 212, 107582-107582 (2020).
PubMed: 32707235

Assembly members:

Assembly members:
entity_1, polymer, . residues, Formula weight is not available
entity_GDP, non-polymer, 443.201 Da.
entity_MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet3a

Data sets:
Data typeCount
13C chemical shifts397
15N chemical shifts136
1H chemical shifts136

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rab4a1
2GDP2
3MG3

Entities:

Entity 1, Rab4a - Formula weight is not available

1   METSERGLUTHRTYRASPPHELEUPHELYS
2   PHELEUVALILEGLYASNALAGLYTHRGLY
3   LYSSERCYSLEULEUHISGLNPHEILEGLU
4   LYSLYSPHELYSASPASPSERASNHISTHR
5   ILEGLYVALGLUPHEGLYSERLYSILEILE
6   ASNVALGLYGLYLYSTYRVALLYSLEUGLN
7   ILETRPASPTHRALAGLYGLNGLUARGPHE
8   ARGSERVALTHRARGSERTYRTYRARGGLY
9   ALAALAGLYALALEULEUVALTYRASPILE
10   THRSERARGGLUTHRTYRASNALALEUTHR
11   ASNTRPLEUTHRASPALAARGMETLEUALA
12   SERGLNASNILEVALILEILELEUCYSGLY
13   ASNLYSLYSASPLEUASPALAASPARGGLU
14   VALTHRPHELEUGLUALASERARGPHEALA
15   GLNGLUASNGLULEUMETPHELEUGLUTHR
16   SERALALEUTHRGLYGLUASPVALGLUGLU
17   ALAPHEVALGLNCYSALAARGLYSILELEU
18   ASNLYSHISHISHISHISHISHIS

Entity 2, GDP - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Entity 3, MG - Mg - 24.305 Da.

1   MG

Samples:

sample_1: GUANOSINE-5'-DIPHOSPHATE, [U-99% 15N], 1 mM; Rab4a, [U-99% 13C; U-99% 15N], 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis v2.4.2 - chemical shift assignment

TOPSPIN v3.6.2 - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks