BMRB Entry 50180

Title:
Cdc42 bound to GTP (AMPylation at T35)
Deposition date:
2020-01-28
Original release date:
2020-05-30
Authors:
Kang, Hyun-Seo; Barthelmes, Katja; Sattler, Michael
Citation:

Citation: Barthelmes, Katja; Ramcke, Evelyn; Kang, Hyun-Seo; Sattler, Michael; Itzen, Aymelt. "Conformational Control of Small GTPases by AMPylation"  Proc. Natl. Acad. Sci. U.S.A. 117, 5772-5781 (2020).
PubMed: 32123090

Assembly members:

Assembly members:
entity_1_Cdc42, polymer, 188 residues, Formula weight is not available
entity_GTP, non-polymer, 523.180 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pOPINM

Data sets:
Data typeCount
15N chemical shifts162
1H chemical shifts171

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cdc421
2GTP2

Entities:

Entity 1, Cdc42 188 residues - Formula weight is not available

AMPylation at T35

1   HISMETGLNTHRILELYSCYSVALVALVAL
2   GLYASPGLYALAVALGLYLYSTHRCYSLEU
3   LEUILESERTYRTHRTHRASNLYSPHEPRO
4   SERGLUTYRVALPROTHRVALPHEASPASN
5   TYRALAVALTHRVALMETILEGLYGLYGLU
6   PROTYRTHRLEUGLYLEUPHEASPTHRALA
7   GLYLEUGLUASPTYRASPARGLEUARGPRO
8   LEUSERTYRPROGLNTHRASPVALPHELEU
9   VALCYSPHESERVALVALSERPROSERSER
10   PHEGLUASNVALLYSGLULYSTRPVALPRO
11   GLUILETHRHISHISCYSPROLYSTHRPRO
12   PHELEULEUVALGLYTHRGLNILEASPLEU
13   ARGASPASPPROSERTHRILEGLULYSLEU
14   ALALYSASNLYSGLNLYSPROILETHRPRO
15   GLUTHRALAGLULYSLEUALAARGASPLEU
16   LYSALAVALLYSTYRVALGLUCYSSERALA
17   LEUTHRGLNLYSGLYLEULYSASNVALPHE
18   ASPGLUALAILELEUALAALALEUGLUPRO
19   PROGLUPROLYSLYSPROLYSLYS

Entity 2, GTP - C10 H16 N5 O14 P3 - 523.180 Da.

1   GTP

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 0.3 mM; HEPES 20 mM; DTE 2 mM; sodium chloride 50 mM; MgCl2 1 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks