BMRB Entry 50142

Title:
Nb-SUMO1
Deposition date:
2019-12-30
Original release date:
2020-08-14
Authors:
Chatterjee, Kiran Sankar; Das, Ranabir
Citation:

Citation: Nair, Ashwin; Chatterjee, Kiran Sankar; Jha, Vikram; Das, Ranabir; Shivaprasad, P. "Stability of Begomoviral pathogenicity determinant betaC1 is modulated by mutually antagonistic SUMOylation and SIM interactions"  BMC Biol. 18, 110-110 (2020).
PubMed: 32867776

Assembly members:

Assembly members:
Nb-SUMO1, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 4100   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Nicotiana benthamiana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b

Data sets:
Data typeCount
13C chemical shifts251
15N chemical shifts86
1H chemical shifts86

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nb-SUMO1 monomer1

Entities:

Entity 1, Nb-SUMO1 monomer 114 residues - Formula weight is not available

HIS tagged SUMO1 sequence from Nicotiana benthamiana. Residues -16 to -11 represent HIS tag followed by (-10 to 0) enzymatic cleavage site.

1   METHISHISHISHISHISHISLEUGLUVAL
2   LEUPHEGLNGLYPROLEUGLYSERMETLEU
3   GLNTHRGLUGLUASPLYSLYSPROSERGLY
4   ASPGLNALAALAHISILEASNLEULYSVAL
5   LYSGLYGLNASPGLYASNGLUVALPHEPHE
6   ARGILELYSARGSERTHRGLNLEULYSLYS
7   LEUMETASNALATYRCYSASPARGGLNSER
8   VALASPPHEASNSERILEALAPHELEUPHE
9   ASPGLYARGARGLEUARGALAGLUGLNTHR
10   PROASPGLULEUGLUMETGLUASPGLYASP
11   GLUILEASPALAMETLEUHISGLNTHRGLY
12   GLYSERTHRVAL

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 1.1 mM

sample_conditions_1: ionic strength: 0.162 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

NMRPipe, Bahrami, Markley, Assadi, and Eghbalnia, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, collection, peak picking, processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks