BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50077

Title: 1H, 15N and 13C assignment of BRCA2 48-218(C4A)   PubMed: 31900740

Deposition date: 2019-10-23 Original release date: 2019-10-29

Authors: Julien, Manon; Miron, Simona; Carreira, Aura; Theillet, Francois-Xavier; Zinn-Justin, Sophie

Citation: Julien, Manon; Miron, Simona; Carreira, Aura; Theillet, Francois-Xavier; Zinn-Justin, Sophie. "1H, 13C and 15N backbone resonance assignment of the human BRCA2 N-terminal region"  Biomol. NMR Assignments 14, 79-85 (2020).

Assembly members:
entity_1, polymer, 172 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: GEESEHKNNNYEPNLFKTPQ RKPSYNQLASTPIIFKEQGL TLPLYQSPVKELDKFKLDLG RNVPNSRHKSLRTVKTKMDQ ADDVSAPLLNSALSESPVVL QATHVTPQRDKSVVAGSLFH TPKFVKGRQTPKHISESLGA EVDPDMSWSSSLATPPTLSS TVLIVRNEEASE

Data sets:
Data typeCount
13C chemical shifts496
15N chemical shifts152
1H chemical shifts152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BRCA2 48-218(C4A)1

Entities:

Entity 1, BRCA2 48-218(C4A) 172 residues - Formula weight is not available

1   GLYGLUGLUSERGLUHISLYSASNASNASN
2   TYRGLUPROASNLEUPHELYSTHRPROGLN
3   ARGLYSPROSERTYRASNGLNLEUALASER
4   THRPROILEILEPHELYSGLUGLNGLYLEU
5   THRLEUPROLEUTYRGLNSERPROVALLYS
6   GLULEUASPLYSPHELYSLEUASPLEUGLY
7   ARGASNVALPROASNSERARGHISLYSSER
8   LEUARGTHRVALLYSTHRLYSMETASPGLN
9   ALAASPASPVALSERALAPROLEULEUASN
10   SERALALEUSERGLUSERPROVALVALLEU
11   GLNALATHRHISVALTHRPROGLNARGASP
12   LYSSERVALVALALAGLYSERLEUPHEHIS
13   THRPROLYSPHEVALLYSGLYARGGLNTHR
14   PROLYSHISILESERGLUSERLEUGLYALA
15   GLUVALASPPROASPMETSERTRPSERSER
16   SERLEUALATHRPROPROTHRLEUSERSER
17   THRVALLEUILEVALARGASNGLUGLUALA
18   SERGLU

Samples:

sample_1: DSS 50 uM; D2O, [U-99% 2H], 5%; BRCA2 48-218(C4A), [U-95% 13C; U-95% 15N], 0.25 mM; HEPES 50 mM; EDTA 1 mM; DTT 2 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.4; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4, Bruker Biospin, CCPN - chemical shift assignment, collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP P51587

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts