BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4993

Title: Spatial structure of Zervamicin IIB bound to DPC micelles.

Authors: Shenkarev, Zakhar; Balashova, Tamara; Efremov, Roman; Yakimenko, Zoya; Ovchinnikova, Tatyana; Raap, Jan; Arseniev, Alexander

Citation: Shenkarev, Zakhar; Balashova, Tamara; Efremov, Roman; Yakimenko, Zoya; Ovchinnikova, Tatyana; Raap, Jan; Arseniev, Alexander. "Spatial Structure of Zervamicin IIB bound to DPC Micelles: Implications for Voltage-gating"  Biophys. J. 82, 762-771 (2002).

Assembly members:
Zervamicin IIB, polymer, 17 residues, 1838 Da.

Natural source:   Common Name: Emericellopsis salmosynnemata   Taxonomy ID: 118885   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Emericellopsis salmosynnemata

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
Zervamicin IIB: XWIQXITXLXXQXXXPX

Data sets:
Data typeCount
13C chemical shifts56
15N chemical shifts16
1H chemical shifts127
coupling constants8

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Zervamicin IIB1

Entities:

Entity 1, Zervamicin IIB 17 residues - 1838 Da.

1   ACETRPILEGLNDIVILETHRAIBLEUAIB
2   HYPGLNAIBHYPAIBPROPHL

Samples:

Sample_1: Zervamicin IIB 1.8 mM; DPC 70 mM

Ex-cond_1: pH: 6.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYSample_1not availableEx-cond_1
2D 1H-1H NOESYSample_1not availableEx-cond_1
2D 1H-1H DQF-COSYSample_1not availableEx-cond_1

Software:

VNMR v5.3 - aquisition, processing

XEASY v2.1 - assignment, integration

DYANA v1.5 - structure calculation

DISCOVER vINSIGHT II/95 - refinement

NMR spectrometers:

  • Varian Unity 600 MHz

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