BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4992

Title: Assignment of 1H, 13C and 15N NMR signals from the toluene 4-monooxygenase Rieske ferredoxin

Authors: Luo, Wei; Moe, Luke; Skjeldal, Lars; Pikus, Jeremie; Markley, John; Fox, Brian

Citation: Luo, Wei; Moe, Luke; Skjeldal, Lars; Pikus, Jeremie; Markley, John; Fox, Brian. "Letter to the Editor: Assignment of 1H, 13C and 15N NMR signals from toluene 4-monooxygenase Rieske ferredoxin in its oxidized state"  J. Biomol. NMR 21, 73-74 (2001).

Assembly members:
ferredoxin, polymer, 112 residues, 12300 Da.
FES, non-polymer, 175.820 Da.

Natural source:   Common Name: Pseudomonas mendocina   Taxonomy ID: 300   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas mendocina

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
ferredoxin: MSFEKICSLDDIWVGEMETF ETSDGTEVLIVNSEEHGVKA YQAMCPHQEILLSEGSYEGG VITCRAHLWTFNDGTGHGIN PDDCCLAEYPVEVKGDDIYV STKGILPNKAHS

Data sets:
Data typeCount
1H chemical shifts606
13C chemical shifts373
15N chemical shifts102

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1T4moC monomer1
2Iron/Sulfur cluster2

Entities:

Entity 1, T4moC monomer 112 residues - 12300 Da.

1   METSERPHEGLULYSILECYSSERLEUASP
2   ASPILETRPVALGLYGLUMETGLUTHRPHE
3   GLUTHRSERASPGLYTHRGLUVALLEUILE
4   VALASNSERGLUGLUHISGLYVALLYSALA
5   TYRGLNALAMETCYSPROHISGLNGLUILE
6   LEULEUSERGLUGLYSERTYRGLUGLYGLY
7   VALILETHRCYSARGALAHISLEUTRPTHR
8   PHEASNASPGLYTHRGLYHISGLYILEASN
9   PROASPASPCYSCYSLEUALAGLUTYRPRO
10   VALGLUVALLYSGLYASPASPILETYRVAL
11   SERTHRLYSGLYILELEUPROASNLYSALA
12   HISSER

Entity 2, Iron/Sulfur cluster - Fe2 S2 - 175.820 Da.

1   FES

Samples:

sample_1: toluene 4-monooxygenase, [U-15N; U-13C], 4.9 mM

Ex-cond_1: pH: 6.4; temperature: 298 K; ionic strength: 60 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
1N-15N HSQCsample_1not availableEx-cond_1
1H-15N-1H TOCSYsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HCACOsample_1not availableEx-cond_1
HCCH COSYsample_1not availableEx-cond_1
H(CCO)NH TOCSYsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
C(CO)NHsample_1not availableEx-cond_1
sidechain HNCOsample_1not availableEx-cond_1
sidechain HNCACBsample_1not availableEx-cond_1

Software:

FELIX v98 - data processing

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

GenBank AAS66662 AAA26001
SWISS-PROT Q00458
PDB