BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4983

Title: Backbone 1H, 15N, 13C and side chain 13C assignment of YUH1-Ub in a 35 kDa complex

Authors: Rajesh, Sundaresan

Citation: Rajesh, Sundaresan. "Backbone 1H,13C,15N and side chain 13C assignment of YUH1-Ub in a 35 kDa covalent complex"  J. Biomol. NMR ., .-..

Assembly members:
Yuh1, polymer, 236 residues, Formula weight is not available
Ubiquitin, polymer, 76 residues, Formula weight is not available

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Yuh1: MSGENRAVVPIESNPEVFTN FAHKLGLKNEWAYFDIYSLT EPELLAFLPRPVKAIVLLFP INEDRKSSTSQQITSSYDVI WFKQSVKNACGLYAILHSLS NNQSLLEPGSDLDNFLKSQS DTSSSKNRFDDVTTDQFVLN VIKENVQTFSTGQSEAPEAT ADTNLHYITYVEENGGIFEL DGRNLSGPLYLGKSDPTATD LIEQELVRVRVASYMENANE EDVLNFAMLGLGPNWE
Ubiquitin: MQIFVKTLTGKTITLEVEPS DTIDNVKSKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGX

Data sets:
Data typeCount
1H chemical shifts293
15N chemical shifts293
13C chemical shifts1182

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YUH1 monomer1
2Ubiquitin2

Entities:

Entity 1, YUH1 monomer 236 residues - Formula weight is not available

1   METSERGLYGLUASNARGALAVALVALPRO
2   ILEGLUSERASNPROGLUVALPHETHRASN
3   PHEALAHISLYSLEUGLYLEULYSASNGLU
4   TRPALATYRPHEASPILETYRSERLEUTHR
5   GLUPROGLULEULEUALAPHELEUPROARG
6   PROVALLYSALAILEVALLEULEUPHEPRO
7   ILEASNGLUASPARGLYSSERSERTHRSER
8   GLNGLNILETHRSERSERTYRASPVALILE
9   TRPPHELYSGLNSERVALLYSASNALACYS
10   GLYLEUTYRALAILELEUHISSERLEUSER
11   ASNASNGLNSERLEULEUGLUPROGLYSER
12   ASPLEUASPASNPHELEULYSSERGLNSER
13   ASPTHRSERSERSERLYSASNARGPHEASP
14   ASPVALTHRTHRASPGLNPHEVALLEUASN
15   VALILELYSGLUASNVALGLNTHRPHESER
16   THRGLYGLNSERGLUALAPROGLUALATHR
17   ALAASPTHRASNLEUHISTYRILETHRTYR
18   VALGLUGLUASNGLYGLYILEPHEGLULEU
19   ASPGLYARGASNLEUSERGLYPROLEUTYR
20   LEUGLYLYSSERASPPROTHRALATHRASP
21   LEUILEGLUGLNGLULEUVALARGVALARG
22   VALALASERTYRMETGLUASNALAASNGLU
23   GLUASPVALLEUASNPHEALAMETLEUGLY
24   LEUGLYPROASNTRPGLU

Entity 2, Ubiquitin 76 residues - Formula weight is not available

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEASPASNVALLYSSERLYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLZ

Samples:

sample_1: Yuh1, [U-95% 2H; U-13C; U-15N], 2 mM; Ubiquitin, [U-95% 2H; U-13C; U-15N], 2 mM

Cond_1: pH: 6.0; temperature: 303 K; ionic strength: 0.10 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableCond_1
HNCOsample_1not availableCond_1
HN(CA)COsample_1not availableCond_1
HNCAsample_1not availableCond_1
HN(CO)CAsample_1not availableCond_1
HNCACBsample_1not availableCond_1
HN(COCA)CBsample_1not availableCond_1
CCNNHsample_1not availableCond_1
CC(CO)NNHsample_1not availableCond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15047 15410 15592 15866 15907 16582 16626 16763 16880 16885 17024 17059 17181 17239 17333 17439 17769 17919 18582 18583 18584 18610 18611 18737 19394 19399 19406 19412 19447 25070 25230 4245 4375 4769 5387 6457 6466 6470 6488 68 7111
PDB
DBJ BAA03983 BAA11842 BAA11843 BAA23632 BAA76889
EMBL CAA07773 CAA11267 CAA21278 CAA28495 CAA29195 CAK44245.1 CAB90826.1 CAB90826.1 CAI77901.1
GB AAA03351 AAA28997 AAA28998 AAA28999 AAA29001
PIR I50437 I51568 I65237 S13928 S21083
PRF 1212243A 1212243C 1212243J 1604470A 1911411A
REF NP_001005123 NP_001006688 NP_001009117 NP_001009286 NP_001013290 XP_001643983.1 XP_001643983.1 XP_001643983.1 XP_001643983.1 XP_001645736.1
SP P05759 P0C016 P0C224 P0C273 P0C275
TPD FAA00316 FAA00317 FAA00318 FAA00319 FAA00318.1 FAA00316.1 FAA00316.1 FAA00316.1 FAA00316.1
TPE CBF76581 CBF85986
TPG DAA08405 DAA09244 DAA09283 DAA09489 DAA18802
GenBank AAD44041.1 AAA36787.1 ABC94632.1 EDM13556.1 EDO17878.1