BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4968

Title: Bovine Pancreatic Trypsin Inhibitor pH 5.8

Authors: Monleon, Daniel; Baran, Michael; Montelione, Gaetano; Sahasrabudhe, Parag

Citation: Monleon, Daniel; Moseley, Hunter; Colson, Kimberly; Anklin, Clemens; Oswald, Robert; Szyperski, Thomas; Montelione, Gaetano. "Rapid Data Collection and Analysis of Protein Resonance Assignments Using AutoProc, AutoPeak, and AutoAssign Software"  J. Struct. Funct. Genomics ., .-..

Assembly members:
BPTI, polymer, 58 residues, Formula weight is not available

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
BPTI: RPDFCLEPPYTGPCKARIIR YFYNAKAGLCQTFVYGGCRA KRNNFKSAEDCMRTCGGA

Data sets:
Data typeCount
13C chemical shifts119
15N chemical shifts52
1H chemical shifts358

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BPTI1

Entities:

Entity 1, BPTI 58 residues - Formula weight is not available

1   ARGPROASPPHECYSLEUGLUPROPROTYR
2   THRGLYPROCYSLYSALAARGILEILEARG
3   TYRPHETYRASNALALYSALAGLYLEUCYS
4   GLNTHRPHEVALTYRGLYGLYCYSARGALA
5   LYSARGASNASNPHELYSSERALAGLUASP
6   CYSMETARGTHRCYSGLYGLYALA

Samples:

Sample_1: BPTI, [U-100% 13C; U-100% 15N], 0.9 mM; D2O 5%; NaOAc 20 mM; CaCl2 25 mM; NaN3 0.02%

BPTI_cond_1: pH: 5.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
N15 HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available
HC(CO)CH TOCSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • VARIAN INOVA 500 MHz
  • VARIAN INOVA 600 MHz

Related Database Links:

BMRB 1039 1156 1179 236 237 262 263 264 338 411 412 413 414 415 416 419 45 46 48 485 4868 49 5171 5307 5358 5359
PDB
EMBL CAA27062 CAA27063 CAA28371 CAA28886 CAA37967
GenBank AAA72535 AAB25189 AAD13685
PRF 1405218A 1405218D 1510193A 681071A
SWISS-PROT P00974