BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4958

Title: 1H, 13C and 15N Sequence-specific Resonance Assignment of the PSCD4 Domain of Diatom cell wall Protein Pleuralin-1

Authors: Wenzler, Michael; Brunner, Eike; Kroger, Nils; Lehmann, Gerhard; Sumper, Manfred; Kalbitzer, Hans; De Sanctis, Silvia; Malloni, Wilhelm

Citation: Wenzler, Michael; Brunner, Eike; Kroger, Nils; Lehmann, Gerhard; Sumper, Manfred; Kalbitzer, Hans. "Letter to the Editor: 1H, 13C and 15N Sequence-specific Resonance Assignment of the PSCD4 Domain of Diatom cell wall Protein Pleuralin-1"  J. Biomol. NMR 20, 191-192 (2001).

Assembly members:
pleuralin-1, formerly HEP200, polymer, 112 residues, 11946 Da.

Natural source:   Common Name: Cylindrotheca fusiformis   Taxonomy ID: 2853   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Cylindrotheca fusiformis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
pleuralin-1, formerly HEP200: SYYHHHHHHTMMPSPEPSSQ PSDCGEVIEECPIDACFLPK SDSARPPDCTAVGRPDCNVL PFPNNIGCPSCCPFECSPDN PMFTPSPDGSPPNCSPTMLP SPSPSAVTVPLT

Data sets:
Data typeCount
13C chemical shifts391
15N chemical shifts86
1H chemical shifts597

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1His6PSCD41

Entities:

Entity 1, His6PSCD4 112 residues - 11946 Da.

1   SERTYRTYRHISHISHISHISHISHISTHR
2   METMETPROSERPROGLUPROSERSERGLN
3   PROSERASPCYSGLYGLUVALILEGLUGLU
4   CYSPROILEASPALACYSPHELEUPROLYS
5   SERASPSERALAARGPROPROASPCYSTHR
6   ALAVALGLYARGPROASPCYSASNVALLEU
7   PROPHEPROASNASNILEGLYCYSPROSER
8   CYSCYSPROPHEGLUCYSSERPROASPASN
9   PROMETPHETHRPROSERPROASPGLYSER
10   PROPROASNCYSSERPROTHRMETLEUPRO
11   SERPROSERPROSERALAVALTHRVALPRO
12   LEUTHR

Samples:

unlabeled: pleuralin-1, formerly HEP200 0.8 mM

15N_labeled: pleuralin-1, formerly HEP200, [U-97% 15N], 0.8 mM

13C_15N_labeled: pleuralin-1, formerly HEP200, [U-97% 13C; U-98% 15N], 0.8 mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
1H-15N-HSQCnot availablenot availableEx-cond_1
1H-15N-NOESY HSQCnot availablenot availableEx-cond_1
1H-15N-TOCSY HSQCnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HACACOnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

EMBL CAA75360.1