BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4942

Title: 1H, 15N and 13C Resonance Assignments and Secondary Structure of the Liver Ribonuclease from Bullfrog Rana catesbeiana

Deposition date: 2001-01-18 Original release date: 2002-04-03

Authors: Su, Ning-Yuan; Liao, You-Di; Chang, Chi-Fon; Wanga, Iren; Chen, Chinpan

Citation: Su, Ning-Yuan; Liao, You-Di; Chang, Chi-Fon; Wanga, Iren; Chen, Chinpan. "Letter to the Editor: 1H, 15N and 13C Resonance Assignments and Secondary Structure of the Liver Ribonuclease from Bullfrog Rana catesbeiana"  J. Biomol. NMR 20, 189-190 (2001).

Assembly members:
rRC-RNase L1[M(-1),Q1], polymer, 112 residues, 12531 Da.

Natural source:   Common Name: bullfrog   Taxonomy ID: 8400   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rana catesbeiana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
rRC-RNase L1[M(-1),Q1]: MQNWAKFKEKHITSTSSIDC NTIMDKAIYIVGGKCKERNT FIISSEDNVKAICSGVSPDR KELSTTSFKLNTCIRDSITP RPCPYHPSPDNNKICVKCEK QLPVHFVGIGKC

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts491
15N chemical shifts116
1H chemical shifts774

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rRC-RNase L11

Entities:

Entity 1, rRC-RNase L1 112 residues - 12531 Da.

1   METGLNASNTRPALALYSPHELYSGLULYS
2   HISILETHRSERTHRSERSERILEASPCYS
3   ASNTHRILEMETASPLYSALAILETYRILE
4   VALGLYGLYLYSCYSLYSGLUARGASNTHR
5   PHEILEILESERSERGLUASPASNVALLYS
6   ALAILECYSSERGLYVALSERPROASPARG
7   LYSGLULEUSERTHRTHRSERPHELYSLEU
8   ASNTHRCYSILEARGASPSERILETHRPRO
9   ARGPROCYSPROTYRHISPROSERPROASP
10   ASNASNLYSILECYSVALLYSCYSGLULYS
11   GLNLEUPROVALHISPHEVALGLYILEGLY
12   LYSCYS

Samples:

sample_1: rRC-RNase L1[M(-1),Q1], [U-15N; U-13C], 1.6 mM

condition_1: pH: 3.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D HBHA(CO)NHnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HN(CA)COnot availablenot availablenot available
3D 15N NOESY-HSQCnot availablenot availablenot available
3D 15N TOCSY-HSQCnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
3D C(CO)NHnot availablenot availablenot available

Software:

xwinnmr v2.6 - data processing

AURELIA - assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

GenBank AAG30414
SWISS-PROT P14626

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts