BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4936

Title: 1H, 13C and 15N chemical shift assignments of the outer membrane protein OmpX from E.coli in DHPC micelles   PubMed: 11226244

Authors: Fernandez, Cesar; Adeishvili, Koba; Wuthrich, Kurt

Citation: Fernandez, Cesar; Adeishvili, Koba; Wuthrich, Kurt. "Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles."  Proc. Natl. Acad. Sci. U. S. A. 98, 2358-2363 (2001).

Assembly members:
Outer membrane protein X, polymer, 148 residues, Formula weight is not available

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Outer membrane protein X: ATSTVTGGYAQSDAQGQMNK MGGFNLKYRYEEDNSPLGVI GSFTYTEKSRTASSGDYNKN QYYGITAGPAYRINDWASIY GVVGVGYGKFQTTEYPTYKH DTTDYGFSYGAGLQFNPMEN VALDFSYEQSRIRSVDVGTW IAGVGYRF

Data sets:
Data typeCount
1H chemical shifts140
13C chemical shifts407
15N chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1OmpX1

Entities:

Entity 1, OmpX 148 residues - Formula weight is not available

1   ALATHRSERTHRVALTHRGLYGLYTYRALA
2   GLNSERASPALAGLNGLYGLNMETASNLYS
3   METGLYGLYPHEASNLEULYSTYRARGTYR
4   GLUGLUASPASNSERPROLEUGLYVALILE
5   GLYSERPHETHRTYRTHRGLULYSSERARG
6   THRALASERSERGLYASPTYRASNLYSASN
7   GLNTYRTYRGLYILETHRALAGLYPROALA
8   TYRARGILEASNASPTRPALASERILETYR
9   GLYVALVALGLYVALGLYTYRGLYLYSPHE
10   GLNTHRTHRGLUTYRPROTHRTYRLYSHIS
11   ASPTHRTHRASPTYRGLYPHESERTYRGLY
12   ALAGLYLEUGLNPHEASNPROMETGLUASN
13   VALALALEUASPPHESERTYRGLUGLNSER
14   ARGILEARGSERVALASPVALGLYTHRTRP
15   ILEALAGLYVALGLYTYRARGPHE

Samples:

sample: Outer membrane protein X, [U-98% 13C; U-98% 15N; U-2H], 2.0 mM; DHPC 300 mM; H2O 97%; D2O 3%; KPO4 20 mM; NaCl 100 mM

exp_conditions: pH: 6.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCAsamplenot availableexp_conditions
HN(CO)CAsamplenot availableexp_conditions
HNCACBsamplenot availableexp_conditions
3D [1H,1H]-NOESY-[15N,1H]-TROSYsamplenot availableexp_conditions

Software:

XEASY -

NMR spectrometers:

  • Bruker DRX 750 MHz

Related Database Links:

BMRB 15201 18796 18797
PDB
DBJ BAA35486 BAB34315 BAG76394 BAI24257 BAI29701
EMBL CAP75284 CAQ31315 CAQ97717 CAR02170 CAR06985
GB AAA21856 AAA66329 AAC73901 AAG55186 AAN42399
REF NP_286578 NP_308919 NP_415335 NP_706692 NP_752830
SP P0A917 P0A918 P0A919 P0A920