BMRB Entry 4901

Title:
1H, 15N, 13C assignments of the N-terminal domain of the human TFIIH p62 subunit
Deposition date:
2000-11-22
Original release date:
2001-04-26
Authors:
Gervais, Virginie; Lamour, Valerie; Gaudin, Florence; Thierry, Jean-Claude; Kieffer, Bruno
Citation:

Citation: Gervais, Virginie; Lamour, Valerie; Gaudin, Florence; Thierry, Jean-Claude; Kieffer, Bruno. "Letter to the Editor: 1H, 15N, 13C resonances of the N-terminal domain of the human TFIIH p62 subunit "  J. Biomol. NMR 19, 281-282 (2001).

Assembly members:

Assembly members:
human TFIIH p62 subunit, polymer, 110 residues, 12432 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts729
13C chemical shifts316
15N chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p62 monomer1

Entities:

Entity 1, p62 monomer 110 residues - 12432 Da.

1   GLYSERMETALATHRSERSERGLUGLUVAL
2   LEULEUILEVALLYSLYSVALARGGLNLYS
3   LYSGLNASPGLYALALEUTYRLEUMETALA
4   GLUARGILEALATRPALAPROGLUGLYLYS
5   ASPARGPHETHRILESERHISMETTYRALA
6   ASPILELYSCYSGLNLYSILESERPROGLU
7   GLYLYSALALYSILEGLNLEUGLNLEUVAL
8   LEUHISALAGLYASPTHRTHRASNPHEHIS
9   PHESERASNGLUSERTHRALAVALLYSGLU
10   ARGASPALAVALLYSASPLEULEUGLNGLN
11   LEULEUPROLYSPHELYSARGLYSALAASN

Samples:

sample_1: human TFIIH p62 subunit, [13C; U-98% 15N], 1.5 mM

sample_2: human TFIIH p62 subunit 2.4 mM

cond_1: pH: 7.4; temperature: 293 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablecond_1
HNCAnot availablenot availablecond_1
HN(CO)CAnot availablenot availablecond_1
CBCA(CO)NHnot availablenot availablecond_1
HNCACBnot availablenot availablecond_1
15N-TOCSY-HSQCnot availablenot availablecond_1
15N-NOESY-HSQCnot availablenot availablecond_1
HBHA(CO)NHnot availablenot availablecond_1
C(CO)NHnot availablenot availablecond_1
H(CCO)NHnot availablenot availablecond_1
HCCH-TOCSYnot availablenot availablecond_1
2D NOESYnot availablenot availablecond_1
2D TOCSYnot availablenot availablecond_1

Software:

FELIX v95 - spectra processing

XEASY v1.3.10 - spectra assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ BAB15621 BAB23789 BAE02479 BAG10896 BAG35811
EMBL CAA05340 CAG33649
GB AAA58399 AAB23294 AAH00365 AAH04452 AAH52837
REF NP_001039615 NP_001101955 NP_001135779 NP_001186521 NP_001271594
SP P32780 Q9DBA9
TPG DAA13968
AlphaFold Q9DBA9 P32780

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks