BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4870

Title: Sequence-specific 1H, 15N, and 13C resonance assignments for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit

Authors: Poznanski, Jaroslaw; Zhukow, Igor; Bolewska, Krystyna; Wierzchowski, Kazimierz

Citation: Poznanski, Jaroslaw; Zhukov, Igor; Bolewska, Krystyna; Wierzchowski, Kazimierz. "Letter to the Editor: Sequence-specific 1H, 15N, and 13C resonance assignments for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit"  J. Biomol. NMR 20, 181-182 (2001).

Assembly members:
region 4 of sigma70 of E. coli RNA polymerase, polymer, 106 residues, 12164 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
region 4 of sigma70 of E. coli RNA polymerase: GSSHHHHHHSSGLVPRGSHM LELPLDSATTESLRAATHDV LAGLTAREAKVLRMRFGIDM NTDYTLEEVGKQFDVTRERI RQIEAKALRKLRHPSRSEVL RSFLDD

Data sets:
Data typeCount
1H chemical shifts681
13C chemical shifts435
15N chemical shifts108
coupling constants66
T1 relaxation values94
T2 relaxation values94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
14 sigma701

Entities:

Entity 1, 4 sigma70 106 residues - 12164 Da.

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYLEUVALPROARGGLYSERHISMET
3   LEUGLULEUPROLEUASPSERALATHRTHR
4   GLUSERLEUARGALAALATHRHISASPVAL
5   LEUALAGLYLEUTHRALAARGGLUALALYS
6   VALLEUARGMETARGPHEGLYILEASPMET
7   ASNTHRASPTYRTHRLEUGLUGLUVALGLY
8   LYSGLNPHEASPVALTHRARGGLUARGILE
9   ARGGLNILEGLUALALYSALALEUARGLYS
10   LEUARGHISPROSERARGSERGLUVALLEU
11   ARGSERPHELEUASPASP

Samples:

sample_1: region 4 of sigma70 of E. coli RNA polymerase, [U-13C; U-15N], 3 mM

cond_1: pH: 2.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablenot available
2D 1H-13C HSQCnot availablenot availablenot available
3D C(CO)NHnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D CBCANHnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D (HCA)CO(CA)NHnot availablenot availablenot available
2D J-MODULATED 1H-15N HSQCnot availablenot availablenot available
2D H(N)COnot availablenot availablenot available
3D HA(CO)NHnot availablenot availablenot available
3D H(CO)NHnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available

Software:

nmrpipe v1.7 - data processing

XEasy v1.3.11 - peak assignment, sequential assignment

NMR spectrometers:

  • Varian Unity Plus 500 MHz

Related Database Links:

BMRB 15930 15936 15975
PDB
DBJ BAB37373 BAE77118 BAG66772 BAG78873 BAH65247
EMBL CAD07736 CAL34114 CAP77541 CAQ33404 CAQ90501
GB AAA24601 AAA27242 AAA89147 AAB60181 AAC76103
PIR AB0893
REF NP_289642 NP_311977 NP_417539 NP_457602 NP_462126
SP P00579 P0A2E3 P0A2E4