BMRB Entry 4837

Title:
Solution Structure of Methylophilus methylotrophus Cytochrome C'': Insights into the Structural Basis of Heme-ligand Detachment
Deposition date:
2000-09-20
Original release date:
2001-07-11
Authors:
Brennan, L.; Turner, D.; Fareleira, P.; Santos, H.
Citation:

Citation: Brennan, L.; Turner, D.; Fareleira, P.; Santos, H.. "Solution Structure of Methylophilus methylotrophus Cytochrome C'': Insights into the Structural Basis of Heme-ligand Detachment"  J. Mol. Biol. 308, 353-365 (2001).
PubMed: 11327772

Assembly members:

Assembly members:
CYTOCHROME C'', polymer, 124 residues, 15000 Da.
PROTOPORPHYRIN IX CONTAINING FE, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 17   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Methylophilus methylotrophus

Experimental source:

Experimental source:   Production method: purified from the natural source

Data sets:
Data typeCount
1H chemical shifts885

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CYTOCHROME C''1
2Heme C2

Entities:

Entity 1, CYTOCHROME C'' 124 residues - 15000 Da.

1   ASPVALTHRASNALAGLULYSLEUVALTYR
2   LYSTYRTHRASNILEALAHISSERALAASN
3   PROMETTYRGLUALAPROSERILETHRASP
4   GLYLYSILEPHEPHEASNARGLYSPHELYS
5   THRPROSERGLYLYSGLUALAALACYSALA
6   SERCYSHISTHRASNASNPROALAASNVAL
7   GLYLYSASNILEVALTHRGLYLYSGLUILE
8   PROPROLEUALAPROARGVALASNTHRLYS
9   ARGPHETHRASPILEASPLYSVALGLUASP
10   GLUPHETHRLYSHISCYSASNASPILELEU
11   GLYALAASPCYSSERPROSERGLULYSALA
12   ASNPHEILEALATYRLEULEUTHRGLUTHR
13   LYSPROTHRLYS

Entity 2, Heme C 1 residues - C34 H32 N4 O4 FE1 - Formula weight is not available

1   HEC_ox

Samples:

sample_1: CYTOCHROME C'' 3.5 mM; ampicillin 70 uM; kanamicine 50 uM; chloramphenicol 50 uM

sample_cond_1: pH: 5.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D-1H-NOESYnot availablenot availablenot available
2D-1H-TOCSYnot availablenot availablenot available
2D-1H-COSYnot availablenot availablenot available
3D-(15N,1H) TOCSY-HSQCnot availablenot availablenot available
3D-(15N,1H) NOESY-HSQCnot availablenot availablenot available

Software:

XEASY -

NMR spectrometers:

  • Varian Unity Plus 750 MHz

Related Database Links:

PDB
GB AAB27865 AAF13764
REF WP_018987197
SP Q9RQB9
AlphaFold Q9RQB9