BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4817

Title: Backbone and side chain 1H, 15N and 13C chemical shifts for Mj0307 from Methanococcus jannascii   PubMed: 11266624

Authors: Cave, John; Cho, Ho; Batchelder, Abigail; Yokota, H.; Kim, R.; Wemmer, David

Citation: Cave, John; Cho, Ho; Batchelder, Abigail; Yokota, H.; Kim, R.; Wemmer, David. "Solution Nuclear Magnetic Resonance Structure of a Protein Disulfide Oxidoreductase from Methanococcus jannaschii"  Protein Sci. 10, 384-396 (2001).

Assembly members:
Mj0307, polymer, 85 residues, 9404 Da.

Natural source:   Common Name: not available   Taxonomy ID: 2190   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Methanococcus jannaschii

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Mj0307: MSKVKIELFTSPMCPHCPAA KRVVEEVANEMPDAVEVEYI NVMENPQKAMEYGIMAVPTI VINGDVEFIGAPTKEALVEA IKKRL

Data sets:
Data typeCount
1H chemical shifts528
13C chemical shifts253
15N chemical shifts72

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Mj03071

Entities:

Entity 1, Mj0307 85 residues - 9404 Da.

1   METSERLYSVALLYSILEGLULEUPHETHR
2   SERPROMETCYSPROHISCYSPROALAALA
3   LYSARGVALVALGLUGLUVALALAASNGLU
4   METPROASPALAVALGLUVALGLUTYRILE
5   ASNVALMETGLUASNPROGLNLYSALAMET
6   GLUTYRGLYILEMETALAVALPROTHRILE
7   VALILEASNGLYASPVALGLUPHEILEGLY
8   ALAPROTHRLYSGLUALALEUVALGLUALA
9   ILELYSLYSARGLEU

Samples:

sample_1: Mj0307 1.5 mM

sample_2: Mj0307, [U-15N], 1.5 mM

sample_3: Mj0307, [U-13C; U-15N], 1.5 mM

condition_1: pH: 6.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-13C NOESY (3D)not availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
CCONHnot availablenot availablenot available
HCCONHnot availablenot availablenot available
HNCACBnot availablenot availablenot available

Software:

Felix v97 - data processing

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAB98293 ACV25141 ADC69759 AIJ05727
REF NP_247280 WP_010869805 WP_012980668 WP_015791875 YP_003128641
SP Q57755