BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4788

Title: 1H,15N, and 13C NMR resonance assignments for the Eps15 Homology domain Resp1

Deposition date: 2000-07-19 Original release date: 2000-12-18

Authors: Kim, Soyoun; Feig, Larry; Baleja, James

Citation: Kim, Soyoun; Dubelman, Ari; Goonesekera, Sunali; Feig, Larry; Baleja, James. "Letter to the Editor: 1H, 15N, and 13C NMR resonance assignments for the Eps15 homology domain of Reps1"  J. Biomol. NMR 18, 367-368 (2000).

Assembly members:
Eps15 homology domain, polymer, 97 residues, 11093 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Eps15 homology domain: ASVGSWKITDEQRQYYVNQF KTIQPDLNGFIPGSAAKEFF TKSKLPILELSHIWELSDFD KDGALTLDEFCAAFHLVVAR KNGYDLPEKLPESLMPK

Data sets:
Data typeCount
1H chemical shifts681
13C chemical shifts286
15N chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Reps1 EH domain1

Entities:

Entity 1, Reps1 EH domain 97 residues - 11093 Da.

1   ALASERVALGLYSERTRPLYSILETHRASP
2   GLUGLNARGGLNTYRTYRVALASNGLNPHE
3   LYSTHRILEGLNPROASPLEUASNGLYPHE
4   ILEPROGLYSERALAALALYSGLUPHEPHE
5   THRLYSSERLYSLEUPROILELEUGLULEU
6   SERHISILETRPGLULEUSERASPPHEASP
7   LYSASPGLYALALEUTHRLEUASPGLUPHE
8   CYSALAALAPHEHISLEUVALVALALAARG
9   LYSASNGLYTYRASPLEUPROGLULYSLEU
10   PROGLUSERLEUMETPROLYS

Samples:

sample_1: Eps15 homology domain, [U-99% 13C; U-99% 15N], 1.3 mM

sample_2: Eps15 homology domain, [U-10% 13C; U-99% 15N], 1.0 mM

sample_3: Eps15 homology domain, [U-15N]-Leu, 0.5 mM

sample_4: Eps15 homology domain, [U-15N]-Phe, 0.5 mM

Ex-cond_1: pH: 6.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCBCAnot availablenot availablenot available
HNCB(CO)CAnot availablenot availablenot available
HNHBnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
CT 1H-13C HSQC experiments were used for stereospecific assignmentnot availablenot availablenot available
of methyl protons of Val and Leu with 10% 13C and 99% 15N labeled sample.not availablenot availablenot available
(sample_2)not availablenot availablenot available

Software:

FELIX v4.2 -

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAC31117 BAE29439 BAE30291 BAG35780
EMBL CAG03521 CAG31901 CDQ63378 CDQ64642
GB AAB94736 AAH12764 AAH21211 AAH87547 AAI33364
REF NP_001012895 NP_001099734 NP_001104535 NP_001122089 NP_001179940
SP O54916 Q96D71
TPG DAA26089

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts