BMRB Entry 4782

Title:
1H, 13C and 15N NMR assignments of the C-type lectin TC14
Deposition date:
2000-07-11
Original release date:
2000-11-29
Authors:
Legge, Glen; Poget, Sebastien; Proctor, Mark; Freund, Stefan; Bycroft, Mark
Citation:

Citation: Legge, Glen; Poget, Sebastien; Proctor, Mark; Freund, Stefan; Bycroft, Mark. "Letter to the Editor: 1H, 13C and 15N NMR assignments of the C-type lectin TC14"  J. Biomol. NMR 18, 283-284 (2000).

Assembly members:

Assembly members:
Polyandrocarpa lectin, polymer, 125 residues, 14031 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 7723   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Polyandrocarpa misakiensis

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts669
13C chemical shifts404
15N chemical shifts125

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TC14 subunit 11
2TC14 subunit 21
3CA 12
4CA 22

Entities:

Entity 1, TC14 subunit 1 125 residues - 14031 Da.

1   METASPTYRGLUILELEUPHESERASPGLU
2   THRMETASNTYRALAASPALAGLYTHRTYR
3   CYSGLNSERARGGLYMETALALEUVALSER
4   SERALAMETARGASPSERTHRMETVALLYS
5   ALAILELEUALAPHETHRGLUVALLYSGLY
6   HISASPTYRTRPVALGLYALAASPASNLEU
7   GLNASPGLYALATYRASNPHELEUTRPASN
8   ASPGLYVALSERLEUPROTHRASPSERASP
9   LEUTRPSERPROASNGLUPROSERASNPRO
10   GLNSERTRPGLNLEUCYSVALGLNILETRP
11   SERLYSTYRASNLEULEUASPASPVALGLY
12   CYSGLYGLYALAARGARGVALILECYSGLU
13   LYSGLULEUASPASP

Entity 2, CA 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Polyandrocarpa lectin 1.5 mM

sample_2: Polyandrocarpa lectin, [U-15N], 1.5 mM

sample_3: Polyandrocarpa lectin, [U-13C; U-15N], 1.5 mM

Ex-cond_1: pH*: 6.0; temperature: 328 K

Experiments:

NameSampleSample stateSample conditions
CBCA(CO)NHnot availablenot availableEx-cond_1
HNCAnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
HBHA(CBCACO)NHnot availablenot availableEx-cond_1
1H-15N TOCSY-HMQCnot availablenot availableEx-cond_1
1H-15N HSQCnot availablenot availableEx-cond_1
1H-13C HSQCnot availablenot availableEx-cond_1
1H-1H DQF-COSYnot availablenot availableEx-cond_1
1H-1H TOCSYnot availablenot availableEx-cond_1
1H-13C HCCH TOCSYnot availablenot availableEx-cond_1

Software:

FELIX v2.30 -

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

SWISS-PROT P16108
DBJ BAB16304 BAA13567
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks